Ex vivo expanded umbilical cord blood T cells maintain naive phenotype and TCR diversity

S. Parmar, S. N. Robinson, K. Komanduri, L. St. John, W. Decker, D. Xing, H. Yang, J. McMannis, R. Champlin, M. de Lima, J. Molldrem, A. Rieber, M. Bonyhadi, R. Berenson, E. J. Shpall

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Background: Umbilical cord blood (CB) is a promising source of hematopoietic stem cells for allogeneic transplantation. However, delayed engraftment and impaired immune reconstitution remain major limitations. Enrichment of donor grafts with CB T cells expanded ex vivo might facilitate improved T-cell immune reconstitution post-transplant. We hypothesized that CB T cells could be expanded using paramagnetic microbeads covalently linked to anti-CD3 and anti-CD28 Ab. Methods: CB units were divided into three fractions: (1) cells cultured without beads, (2) cells cultured with beads and (3) cells cultured with beads following CD3+ magnetic enrichment. All fractions were cultured for 14 days in the presence of IL-2 (200 IU/mL). Results: A mean 100-fold expansion (range 49-154) of total nucleated cells was observed in the CD3+ magnetically enriched fraction. Following expansion, CB T cells retained a naive and/or central memory phenotype and contained a polyclonal TCR diversity demonstrated by spectratyping. Discussion: Our data provide evidence that naive and diverse CB T cells may be expanded ex vivo and warrant additional studies in the setting of human CB transplantation.

Original languageEnglish (US)
Pages (from-to)149-157
Number of pages9
JournalCytotherapy
Volume8
Issue number2
DOIs
StatePublished - May 1 2006
Externally publishedYes

Keywords

  • Cord blood transplant
  • Naive T cells
  • T-cell expansion

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pharmacology

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    Parmar, S., Robinson, S. N., Komanduri, K., St. John, L., Decker, W., Xing, D., Yang, H., McMannis, J., Champlin, R., de Lima, M., Molldrem, J., Rieber, A., Bonyhadi, M., Berenson, R., & Shpall, E. J. (2006). Ex vivo expanded umbilical cord blood T cells maintain naive phenotype and TCR diversity. Cytotherapy, 8(2), 149-157. https://doi.org/10.1080/14653240600620812