Ex vivo evaluation of Tono-Pen and pneumotonometry in cat eyes

Josef Stoiber, Viviana Fernandez, Peggy D. Lamar, Wolfgang Hitzl, Francisco Fantes, Jean Marie Parel

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Purpose: To evaluate the validity and intraobserver reliability of intraocular pressure (IOP) measurements with both pneumotonometry and the Tono-Pen in a closed ex vivo system in cat eyes. Methods: IOP was increased step by step in 5 enucleated cat eyes, while taking IOP measurements with the Tono-Pen and pneumotonometry. The outcomes were compared to readings of a digital manometer simultaneously measuring the actual pressure in the anterior chamber. Results: Pneumotonometry overestimated IOP below 15 mm Hg and underestimated pressures above 20 mm Hg. Tono-Pen tonometry considerably underestimated IOP over the whole spectrum in all of the eyes tested. The pneumotonometer was identified as the more valid and reliable instrument for cat eyes. Conclusion: Both tonometers are clinically useful tools to assess IOP for glaucoma studies using a cat animal model. However, one has to consider underestimation of IOP in the upper ranges. A correction formula can be used to calculate the actual IOP.

Original languageEnglish (US)
Pages (from-to)13-18
Number of pages6
JournalOphthalmic Research
Issue number1
StatePublished - Dec 2005


  • Intraocular pressure measurements, accuracy
  • Pneumotonometry
  • Tono-Pen

ASJC Scopus subject areas

  • Ophthalmology


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