EWSR1-POU5F1 fusion in soft tissue myoepithelial tumors. A molecular analysis of sixty-six cases, including soft tissue, bone, and visceral lesions, showing common involvement of the EWSR1 gene

Cristina R. Antonescu, Lei Zhang, Ning En Chang, Bruce R. Pawel, William Travis, Nora Katabi, Morris Edelman, Andrew Rosenberg, G. Petur Nielsen, Paola Dal Cin, Christopher D M Fletcher

Research output: Contribution to journalArticle

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Abstract

The diagnosis of myoepithelial (ME) tumors outside salivary glands remains challenging, especially in unusual clinical presentations, such as bone or visceral locations. A few reports have indicated EWSR1 gene rearrangement in soft tissue ME tumors, and, in one case each, the fusion partner was identified as either PBX1 or ZNF444. However, larger studies to investigate whether these genetic abnormalities are recurrent or restricted to tumors in soft tissue locations are lacking. Sixty-six ME tumors mainly from soft tissue (71%), but also from skin, bone, and visceral locations, characterized by classic morphological features and supporting immunoprofile were studied. Gene rearrangements in EWSR1, FUS, PBX1, and ZNF444 were investigated by fluorescence in situ hybridization. EWSR1 gene rearrangement was detected in 45% of the cases. A EWSR1-POU5F1 fusion was identified in a pediatric soft tissue tumor by 30Rapid Amplification of cDNA Ends (RACE) and subsequently confirmed in four additional soft tissue tumors in children and young adults. An EWSR1-PBX1 fusion was seen in five cases, whereas EWSR1-ZNF444 and FUS gene rearrangement was noted in one pulmonary tumor each. In conclusion, EWSR1 gene rearrangement is a common event in ME tumors arising outside salivary glands, irrespective of anatomical location. EWSR1-negative tumors were more often benign, superficially located, and showed ductal differentiation, suggesting the possibility of genetically distinct groups. A subset of soft tissue ME tumors with clear cell morphology harbor an EWSR1-POU5F1 fusion, which can be used as a molecular diagnostic test in difficult cases. These findings do not support a pathogenetic relationship between soft tissue ME tumors and their salivary gland counterparts.

Original languageEnglish
Pages (from-to)1114-1124
Number of pages11
JournalGenes Chromosomes and Cancer
Volume49
Issue number12
DOIs
StatePublished - Dec 1 2009
Externally publishedYes

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Myoepithelioma
Gene Rearrangement
Bone and Bones
Genes
Salivary Glands
Neoplasms
Molecular Pathology
Fluorescence In Situ Hybridization
Routine Diagnostic Tests
Young Adult
Complementary DNA
Pediatrics
Lung
Skin

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Medicine(all)

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EWSR1-POU5F1 fusion in soft tissue myoepithelial tumors. A molecular analysis of sixty-six cases, including soft tissue, bone, and visceral lesions, showing common involvement of the EWSR1 gene. / Antonescu, Cristina R.; Zhang, Lei; Chang, Ning En; Pawel, Bruce R.; Travis, William; Katabi, Nora; Edelman, Morris; Rosenberg, Andrew; Nielsen, G. Petur; Dal Cin, Paola; Fletcher, Christopher D M.

In: Genes Chromosomes and Cancer, Vol. 49, No. 12, 01.12.2009, p. 1114-1124.

Research output: Contribution to journalArticle

Antonescu, Cristina R. ; Zhang, Lei ; Chang, Ning En ; Pawel, Bruce R. ; Travis, William ; Katabi, Nora ; Edelman, Morris ; Rosenberg, Andrew ; Nielsen, G. Petur ; Dal Cin, Paola ; Fletcher, Christopher D M. / EWSR1-POU5F1 fusion in soft tissue myoepithelial tumors. A molecular analysis of sixty-six cases, including soft tissue, bone, and visceral lesions, showing common involvement of the EWSR1 gene. In: Genes Chromosomes and Cancer. 2009 ; Vol. 49, No. 12. pp. 1114-1124.
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abstract = "The diagnosis of myoepithelial (ME) tumors outside salivary glands remains challenging, especially in unusual clinical presentations, such as bone or visceral locations. A few reports have indicated EWSR1 gene rearrangement in soft tissue ME tumors, and, in one case each, the fusion partner was identified as either PBX1 or ZNF444. However, larger studies to investigate whether these genetic abnormalities are recurrent or restricted to tumors in soft tissue locations are lacking. Sixty-six ME tumors mainly from soft tissue (71{\%}), but also from skin, bone, and visceral locations, characterized by classic morphological features and supporting immunoprofile were studied. Gene rearrangements in EWSR1, FUS, PBX1, and ZNF444 were investigated by fluorescence in situ hybridization. EWSR1 gene rearrangement was detected in 45{\%} of the cases. A EWSR1-POU5F1 fusion was identified in a pediatric soft tissue tumor by 30Rapid Amplification of cDNA Ends (RACE) and subsequently confirmed in four additional soft tissue tumors in children and young adults. An EWSR1-PBX1 fusion was seen in five cases, whereas EWSR1-ZNF444 and FUS gene rearrangement was noted in one pulmonary tumor each. In conclusion, EWSR1 gene rearrangement is a common event in ME tumors arising outside salivary glands, irrespective of anatomical location. EWSR1-negative tumors were more often benign, superficially located, and showed ductal differentiation, suggesting the possibility of genetically distinct groups. A subset of soft tissue ME tumors with clear cell morphology harbor an EWSR1-POU5F1 fusion, which can be used as a molecular diagnostic test in difficult cases. These findings do not support a pathogenetic relationship between soft tissue ME tumors and their salivary gland counterparts.",
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AU - Zhang, Lei

AU - Chang, Ning En

AU - Pawel, Bruce R.

AU - Travis, William

AU - Katabi, Nora

AU - Edelman, Morris

AU - Rosenberg, Andrew

AU - Nielsen, G. Petur

AU - Dal Cin, Paola

AU - Fletcher, Christopher D M

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AB - The diagnosis of myoepithelial (ME) tumors outside salivary glands remains challenging, especially in unusual clinical presentations, such as bone or visceral locations. A few reports have indicated EWSR1 gene rearrangement in soft tissue ME tumors, and, in one case each, the fusion partner was identified as either PBX1 or ZNF444. However, larger studies to investigate whether these genetic abnormalities are recurrent or restricted to tumors in soft tissue locations are lacking. Sixty-six ME tumors mainly from soft tissue (71%), but also from skin, bone, and visceral locations, characterized by classic morphological features and supporting immunoprofile were studied. Gene rearrangements in EWSR1, FUS, PBX1, and ZNF444 were investigated by fluorescence in situ hybridization. EWSR1 gene rearrangement was detected in 45% of the cases. A EWSR1-POU5F1 fusion was identified in a pediatric soft tissue tumor by 30Rapid Amplification of cDNA Ends (RACE) and subsequently confirmed in four additional soft tissue tumors in children and young adults. An EWSR1-PBX1 fusion was seen in five cases, whereas EWSR1-ZNF444 and FUS gene rearrangement was noted in one pulmonary tumor each. In conclusion, EWSR1 gene rearrangement is a common event in ME tumors arising outside salivary glands, irrespective of anatomical location. EWSR1-negative tumors were more often benign, superficially located, and showed ductal differentiation, suggesting the possibility of genetically distinct groups. A subset of soft tissue ME tumors with clear cell morphology harbor an EWSR1-POU5F1 fusion, which can be used as a molecular diagnostic test in difficult cases. These findings do not support a pathogenetic relationship between soft tissue ME tumors and their salivary gland counterparts.

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