Evolved genetic and phenotypic differences due to mitochondrial-nuclear interactions

Tara Z. Baris, Dominique N. Wagner, David I. Dayan, Xiao Du, Pierre U. Blier, Nicolas Pichaud, Marjorie F Oleksiak, Douglas L Crawford

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The oxidative phosphorylation (OxPhos) pathway is responsible for most aerobic ATP production and is the only pathway with both nuclear and mitochondrial encoded proteins. The importance of the interactions between these two genomes has recently received more attention because of their potential evolutionary effects and how they may affect human health and disease. In many different organisms, healthy nuclear and mitochondrial genome hybrids between species or among distant populations within a species affect fitness and OxPhos functions. However, what is less understood is whether these interactions impact individuals within a single natural population. The significance of this impact depends on the strength of selection for mito-nuclear interactions. We examined whether mito-nuclear interactions alter allele frequencies for ~11,000 nuclear SNPs within a single, natural Fundulus heteroclitus population containing two divergent mitochondrial haplotypes (mt-haplotypes). Between the two mt-haplotypes, there are significant nuclear allele frequency differences for 349 SNPs with a p-value of 1% (236 with 10% FDR). Unlike the rest of the genome, these 349 outlier SNPs form two groups associated with each mt-haplotype, with a minority of individuals having mixed ancestry. We use this mixed ancestry in combination with mt-haplotype as a polygenic factor to explain a significant fraction of the individual OxPhos variation. These data suggest that mito-nuclear interactions affect cardiac OxPhos function. The 349 outlier SNPs occur in genes involved in regulating metabolic processes but are not directly associated with the 79 nuclear OxPhos proteins. Therefore, we postulate that the evolution of mito-nuclear interactions affects OxPhos function by acting upstream of OxPhos.

Original languageEnglish (US)
Article numbere1006517
JournalPLoS Genetics
Volume13
Issue number3
DOIs
StatePublished - Mar 1 2017

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nuclear-cytoplasmic interactions
oxidative phosphorylation
Oxidative Phosphorylation
genome
outlier
ancestry
allele
Haplotypes
Single Nucleotide Polymorphism
haplotypes
health and disease
protein
Gene Frequency
fitness
gene frequency
Fundulidae
Genome
Population
gene
Fundulus heteroclitus

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Baris, T. Z., Wagner, D. N., Dayan, D. I., Du, X., Blier, P. U., Pichaud, N., ... Crawford, D. L. (2017). Evolved genetic and phenotypic differences due to mitochondrial-nuclear interactions. PLoS Genetics, 13(3), [e1006517]. https://doi.org/10.1371/journal.pgen.1006517

Evolved genetic and phenotypic differences due to mitochondrial-nuclear interactions. / Baris, Tara Z.; Wagner, Dominique N.; Dayan, David I.; Du, Xiao; Blier, Pierre U.; Pichaud, Nicolas; Oleksiak, Marjorie F; Crawford, Douglas L.

In: PLoS Genetics, Vol. 13, No. 3, e1006517, 01.03.2017.

Research output: Contribution to journalArticle

Baris TZ, Wagner DN, Dayan DI, Du X, Blier PU, Pichaud N et al. Evolved genetic and phenotypic differences due to mitochondrial-nuclear interactions. PLoS Genetics. 2017 Mar 1;13(3). e1006517. https://doi.org/10.1371/journal.pgen.1006517
Baris, Tara Z. ; Wagner, Dominique N. ; Dayan, David I. ; Du, Xiao ; Blier, Pierre U. ; Pichaud, Nicolas ; Oleksiak, Marjorie F ; Crawford, Douglas L. / Evolved genetic and phenotypic differences due to mitochondrial-nuclear interactions. In: PLoS Genetics. 2017 ; Vol. 13, No. 3.
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