Evolutionary conservation of putative functional domains in the human homolog of the murine His-1 gene

Jie Li, Judith C. Rhodes, David S. Askew

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The mouse His-1 gene encodes a spliced and polyadenylated RNA with no long open reading frame (ORF), making it difficult to distinguish a functional protein coding domain. To identify candidate protein coding ORFs, and other functionally significant regions, we have isolated and sequenced 8.5 kb of a human genomic DNA that is homologous to the mouse His-1 gene. Alignment of the mouse and human sequences required no extensive gapping, indicating that evolutionary constraints have maintained a requirement for colinearity in genomic organization. We have identified the mouse transcriptional start point (tsp) and shown that the sequence of the 5'-flanking region is highly conserved in the human homolog. Sequence comparisons between the mouse and human genes identified conservation of other putative functional domains in exon 3 and in each of the two introns. Southern blot analysis with probes from each of these regions detected homologs in multiple other vertebrate species. However, none of the multiple candidate ORFs in the mouse RNA were conserved in the human sequence, suggesting that the RNA is unlikely to encode a protein. These data suggest that the RNA may be the final and functional product from the mouse His-1 gene.

Original languageEnglish
Pages (from-to)169-176
Number of pages8
JournalGene
Volume184
Issue number2
DOIs
StatePublished - Jan 15 1997
Externally publishedYes

Fingerprint

Genes
Open Reading Frames
RNA
5' Flanking Region
Southern Blotting
Introns
Vertebrates
Exons
Proteins
Messenger RNA
DNA

Keywords

  • Cloning
  • Leukemia
  • Oncogene
  • Retrovirus
  • Sequence

ASJC Scopus subject areas

  • Genetics

Cite this

Evolutionary conservation of putative functional domains in the human homolog of the murine His-1 gene. / Li, Jie; Rhodes, Judith C.; Askew, David S.

In: Gene, Vol. 184, No. 2, 15.01.1997, p. 169-176.

Research output: Contribution to journalArticle

Li, Jie ; Rhodes, Judith C. ; Askew, David S. / Evolutionary conservation of putative functional domains in the human homolog of the murine His-1 gene. In: Gene. 1997 ; Vol. 184, No. 2. pp. 169-176.
@article{ed706fb34e18402bbb92f9a2997060bd,
title = "Evolutionary conservation of putative functional domains in the human homolog of the murine His-1 gene",
abstract = "The mouse His-1 gene encodes a spliced and polyadenylated RNA with no long open reading frame (ORF), making it difficult to distinguish a functional protein coding domain. To identify candidate protein coding ORFs, and other functionally significant regions, we have isolated and sequenced 8.5 kb of a human genomic DNA that is homologous to the mouse His-1 gene. Alignment of the mouse and human sequences required no extensive gapping, indicating that evolutionary constraints have maintained a requirement for colinearity in genomic organization. We have identified the mouse transcriptional start point (tsp) and shown that the sequence of the 5'-flanking region is highly conserved in the human homolog. Sequence comparisons between the mouse and human genes identified conservation of other putative functional domains in exon 3 and in each of the two introns. Southern blot analysis with probes from each of these regions detected homologs in multiple other vertebrate species. However, none of the multiple candidate ORFs in the mouse RNA were conserved in the human sequence, suggesting that the RNA is unlikely to encode a protein. These data suggest that the RNA may be the final and functional product from the mouse His-1 gene.",
keywords = "Cloning, Leukemia, Oncogene, Retrovirus, Sequence",
author = "Jie Li and Rhodes, {Judith C.} and Askew, {David S.}",
year = "1997",
month = "1",
day = "15",
doi = "10.1016/S0378-1119(96)00591-4",
language = "English",
volume = "184",
pages = "169--176",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Evolutionary conservation of putative functional domains in the human homolog of the murine His-1 gene

AU - Li, Jie

AU - Rhodes, Judith C.

AU - Askew, David S.

PY - 1997/1/15

Y1 - 1997/1/15

N2 - The mouse His-1 gene encodes a spliced and polyadenylated RNA with no long open reading frame (ORF), making it difficult to distinguish a functional protein coding domain. To identify candidate protein coding ORFs, and other functionally significant regions, we have isolated and sequenced 8.5 kb of a human genomic DNA that is homologous to the mouse His-1 gene. Alignment of the mouse and human sequences required no extensive gapping, indicating that evolutionary constraints have maintained a requirement for colinearity in genomic organization. We have identified the mouse transcriptional start point (tsp) and shown that the sequence of the 5'-flanking region is highly conserved in the human homolog. Sequence comparisons between the mouse and human genes identified conservation of other putative functional domains in exon 3 and in each of the two introns. Southern blot analysis with probes from each of these regions detected homologs in multiple other vertebrate species. However, none of the multiple candidate ORFs in the mouse RNA were conserved in the human sequence, suggesting that the RNA is unlikely to encode a protein. These data suggest that the RNA may be the final and functional product from the mouse His-1 gene.

AB - The mouse His-1 gene encodes a spliced and polyadenylated RNA with no long open reading frame (ORF), making it difficult to distinguish a functional protein coding domain. To identify candidate protein coding ORFs, and other functionally significant regions, we have isolated and sequenced 8.5 kb of a human genomic DNA that is homologous to the mouse His-1 gene. Alignment of the mouse and human sequences required no extensive gapping, indicating that evolutionary constraints have maintained a requirement for colinearity in genomic organization. We have identified the mouse transcriptional start point (tsp) and shown that the sequence of the 5'-flanking region is highly conserved in the human homolog. Sequence comparisons between the mouse and human genes identified conservation of other putative functional domains in exon 3 and in each of the two introns. Southern blot analysis with probes from each of these regions detected homologs in multiple other vertebrate species. However, none of the multiple candidate ORFs in the mouse RNA were conserved in the human sequence, suggesting that the RNA is unlikely to encode a protein. These data suggest that the RNA may be the final and functional product from the mouse His-1 gene.

KW - Cloning

KW - Leukemia

KW - Oncogene

KW - Retrovirus

KW - Sequence

UR - http://www.scopus.com/inward/record.url?scp=0031023013&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031023013&partnerID=8YFLogxK

U2 - 10.1016/S0378-1119(96)00591-4

DO - 10.1016/S0378-1119(96)00591-4

M3 - Article

C2 - 9031624

AN - SCOPUS:0031023013

VL - 184

SP - 169

EP - 176

JO - Gene

JF - Gene

SN - 0378-1119

IS - 2

ER -