Evidence that transforming growth factor-β is a hormonally regulated negative growth factor in human breast cancer cells

Cornelius Knabbe, Marc E Lippman, Lalage M. Wakefield, Kathleen C. Flanders, Attan Kasid, Rik Derynck, Robert B. Dickson

Research output: Contribution to journalArticle

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Abstract

The hormone-dependent human breast cancer cell line MCF-7 secretes transforming growth factor-β (TGF-β), which can be detected in the culture medium in a biologically active form. These polypeptides compete with human platelet-derived TGF-β for binding to its receptor, are biologically active in TGF-β-specific growth assays, and are recognized and inactivated by TGF-β-specific antibodies. Secretion of active TGF-β is induced 8 to 27-fold under treatment of MCF-7 cells with growth inhibitory concentrations of antiestrogens. Antiestrogen-induced TGF-β from MCF-7 cells inhibits the growth of an estrogen receptor-negative human breast cancer cell line in coculture experiments; growth inhibition is reversed with anti-TGF-β antibodies. We conclude that in MCF-7 cells, TGF-β is a hormonally regulated growth inhibitor with possible autocrine and paracrine functions in breast cancer cells.

Original languageEnglish
Pages (from-to)417-428
Number of pages12
JournalCell
Volume48
Issue number3
DOIs
StatePublished - Feb 13 1987
Externally publishedYes

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Transforming Growth Factors
Intercellular Signaling Peptides and Proteins
Cells
Breast Neoplasms
MCF-7 Cells
Estrogen Receptor Modulators
Growth
Cell Line
Growth Inhibitors
Antibodies
Platelet-Derived Growth Factor
Coculture Techniques
Transforming Growth Factor beta
Estrogen Receptors
Cell growth
Platelets
Culture Media
Assays
Hormones
Peptides

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Knabbe, C., Lippman, M. E., Wakefield, L. M., Flanders, K. C., Kasid, A., Derynck, R., & Dickson, R. B. (1987). Evidence that transforming growth factor-β is a hormonally regulated negative growth factor in human breast cancer cells. Cell, 48(3), 417-428. https://doi.org/10.1016/0092-8674(87)90193-0

Evidence that transforming growth factor-β is a hormonally regulated negative growth factor in human breast cancer cells. / Knabbe, Cornelius; Lippman, Marc E; Wakefield, Lalage M.; Flanders, Kathleen C.; Kasid, Attan; Derynck, Rik; Dickson, Robert B.

In: Cell, Vol. 48, No. 3, 13.02.1987, p. 417-428.

Research output: Contribution to journalArticle

Knabbe, C, Lippman, ME, Wakefield, LM, Flanders, KC, Kasid, A, Derynck, R & Dickson, RB 1987, 'Evidence that transforming growth factor-β is a hormonally regulated negative growth factor in human breast cancer cells', Cell, vol. 48, no. 3, pp. 417-428. https://doi.org/10.1016/0092-8674(87)90193-0
Knabbe, Cornelius ; Lippman, Marc E ; Wakefield, Lalage M. ; Flanders, Kathleen C. ; Kasid, Attan ; Derynck, Rik ; Dickson, Robert B. / Evidence that transforming growth factor-β is a hormonally regulated negative growth factor in human breast cancer cells. In: Cell. 1987 ; Vol. 48, No. 3. pp. 417-428.
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