Evidence that production of interleukin 6 within the rejecting allograft coincides with cytotoxic T lymphocyte development

Henri R. Ford, Rosemary A. Hoffman, David J. Tweardy, Paul Kispert, Stewart Wang, Richard L. Simmons

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

Current evidence suggests that the development of allosensitized cytotoxic T lymphocytes within sponge matrix allografts takes place primarily in situ and may be regulated by the secretory products of the cells infiltrating the graft. In vitro studies have implicated IL-2, IL-4, and IL-6 in CTL development. We have reported that TNF-alpha, macrophage colony-stimulating factor, IL-1, IFN-alpha, and IFN-beta are present in the allograft, but that IL-2 and IL-4 cannot be detected at any time using specific bioassays. In this study, we found significantly higher levels of IL-6 within the allografts compared with the syngeneic grafts. Peak IL-6 activity coincided with the appearance of allosensitized CTL in the allografts. IL-6 concentration in the serum of sponge allografted mice was less than 1% of that found in the graft. The sponge fluid exhibited both hybridoma growth factor and hepatocyte-stimulating factor activities in vitro, and both these activities were neutralized by antibody to murine IL-6 but not by antibody to murine IL-1-beta or TNF-alpha. Messenger RNA for murine IL-6 was detected in the graft-infiltrating cells. The high level of IL-6 found in the allograft coincident with the appearance of cellular immunity suggests that this cytokine might play some role in the development of allospecific CTL in vivo.

Original languageEnglish (US)
Pages (from-to)656-661
Number of pages6
JournalTransplantation
Volume51
Issue number3
DOIs
StatePublished - Mar 1991

ASJC Scopus subject areas

  • Transplantation

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