Evidence that C1s participates in the alternative complement pathway

J. E. Volanakis, D. R. Schultz, R. M. Stroud

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purified C1s, subcomponent of C1, induced electrophoretic conversion of factor B and consumption of hemolytic C3 and C5 in sera genetically deficient in C2 or C4. Blocking of the hemolytic activity of C1s in C2 deficient serum by F(ab')2 anti C1s resulted in inhibition of the alternative pathway, as indicated by the failure of zymosan or cobra venom factor to induce consumption of C3 and C6. Zymosan also failed to activate the alternative pathway when C1s was absorbed from C1r deficient serum using a solid immunoabsorbent. These data, showing that C1s participates in the alternative pathway under certain experimental conditions, suggest the interesting possibility that C1s is important in the activation sequence of both the classical and the alternative pathway more generally.

Original languageEnglish
Pages (from-to)68-80
Number of pages13
JournalInternational Archives of Allergy and Applied Immunology
Volume50
Issue number1
StatePublished - Jan 1 1976
Externally publishedYes

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Alternative Complement Pathway
Zymosan
Serum

ASJC Scopus subject areas

  • Immunology and Allergy

Cite this

Evidence that C1s participates in the alternative complement pathway. / Volanakis, J. E.; Schultz, D. R.; Stroud, R. M.

In: International Archives of Allergy and Applied Immunology, Vol. 50, No. 1, 01.01.1976, p. 68-80.

Research output: Contribution to journalArticle

Volanakis, J. E. ; Schultz, D. R. ; Stroud, R. M. / Evidence that C1s participates in the alternative complement pathway. In: International Archives of Allergy and Applied Immunology. 1976 ; Vol. 50, No. 1. pp. 68-80.
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