Evidence that action potentials activate an internodal potassium conductance in lizard myelinated axons.

1. We have studied action potentials and after-potentials evoked in the internodal region of visualized lizard intramuscular nerve fibres by stimulation of the proximal nerve trunk. Voltage recordings were obtained using microelectrodes inserted into the axon (intra-axonal) or into the layers of myelin (peri-internodal), with the goal of studying conditions required to activate internodal K⁺ currents. 2. Peri-internodal recordings made using K₂SO₄-, KCl- or NaCl-filled electrodes exhibited a negligible resting potential (< 2 mV), but showed action potentials with peak amplitudes of up to 78 mV and a duration less than or equal to that of the intra-axonally recorded action potential. 3. Following ionophoretic application of potassium from a peri-internodal microelectrode, the peri-internodal action potential was followed by a prolonged (hundreds of milliseconds) negative plateau. This plateau was not seen following peri-internodal ionophoresis of sodium. The prolonged negative potential (PNP) was confined to the K⁺-injected internode: it could be recorded by a second peri-internodal microelectrode inserted into the same internode, but not into an adjacent internode. 4. The peri-internodally recorded PNP was accompanied by an equally prolonged intra-axonal depolarizing after-potential, and by an increase in the conductance of the internodal axolemma. However, the K⁺ ionophoresis that produced the PNP had little or no detectable effect on the intra-axonally or peri-internodally recorded resting potential or action potential. These findings suggest that the PNP is generated by an inward current across the axolemma of the K⁺-injected internode, through channels opened following the action potential. 5. Following peri-internodal K⁺ ionophoresis a PNP could also be evoked by passage of depolarizing current pulses through an intra-axonal electrode or by passage of negative current pulses through an electrode in the K⁺-filled peri-internodal region. The threshold for evoking a PNP was less than the threshold for evoking an action potential, and the PNP persisted in 10 μM-tetrodotoxin. Thus the PNP is evoked by depolarization of the axolemma rather than by Na⁺ influx. 6. The PNP was reversibly blocked by tetraethylammonium (TEA, 2-10 mM), but was not blocked by 100 μM-3,4-diaminopyridine or 5 mM-4-aminopyridine. 7. These findings suggest that the PNP is produced by a regenerative inward K⁺ current across the axolemma of an internode, achieved when two conditions are met: first, [K⁺] outside the internode's axolemma is elevated so that the trans-axolemmal electrochemical gradient favours K⁺ influx, and second, the internodal axolemma is depolarized sufficiently to open TEA-sensitive K⁺ channels. 8. Under physiological conditions (normal extracellular [K⁺]) the electrochemical gradient across the internodal axolemma would be expected to favour K⁺ efflux from the axon. Consistent with the hypothesis that the action potential activates an internodal K⁺ conductance, peri-internodal recordings made using microelectrodes filled With NaCl or physiological saline show a brief positive after-potential (suggesting K⁺ efflux from the axon), which is blocked by TEA but not by aminopyridines. Activation of this K⁺ conductance would be expected to limit the peak amplitude of the passive depolarizing after-potential that follows the intra-axonally recorded action potential, and thereby limit progressive depolarization of the axon during high frequency activity.