Abstract
This study evaluates leukocyte infiltration, ICAM-1 expression, permeability across the blood-spinal cord barrier (BSCB)and therapeutic effect of ICAM-1 following epidural administration. Rabbits weighing ( 2.5 Kg) were anesthetized with halothane, an abdominal incision was made, the descending aorta exposed and occluded with an aneusysm clip. After varible periods of occlusion, the clip was removed and incision closed with sutures and skin clips. Sham-operated controls were prepared identically but descending aorta was not occluded. Following 2,4 and 8h after 30 min occlusion the following were evaluated in the lumbar cord: ICAM-1 expression by immunohistochemistry; leukocyte infiltration by electron microscopy; vascular endothelial permeability across BSCB by radiometry. The therapeutic efficacy of ICAM-1 MAb was tested by preemptive epidural administration of 0.5mg/Kg ICAM-1 MAb, following variable occlusion periods, i.e., 10,15.20,25 and 30 min. The neurological examination was performed 20h later on a Tarlov's scale in a blinded fashion. The ICAM-1 expression and leukocyte recruitment/infiltration were significantly increased following 8h after 30 min occlusion. The BSCB permeability of D-mannitol and inulin was significantly increased. The quantal response curve computed as dichotomous function of non-paraplegia/paraplegia Vs occlusion period based on neurologic evaluation, revealed that treatment with ICAM-1 MAb produced significant reduction in neurological deficits (P<0.05)in this model.
| Original language | English |
|---|---|
| Journal | FASEB Journal |
| Volume | 12 |
| Issue number | 5 |
| State | Published - Mar 20 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Cell Biology
Cite this
Evidence for leukocyte infiltration, ICAM-1 expression, permeability following ischemic paraplegia : Functional blocking of ICAM-1 as a new therapeutic target. / Naidu, Kamatham A.; Sutton, E. Truitt; Fu, Eugene; Prockop, Leon D.
In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evidence for leukocyte infiltration, ICAM-1 expression, permeability following ischemic paraplegia
T2 - Functional blocking of ICAM-1 as a new therapeutic target
AU - Naidu, Kamatham A.
AU - Sutton, E. Truitt
AU - Fu, Eugene
AU - Prockop, Leon D.
PY - 1998/3/20
Y1 - 1998/3/20
N2 - This study evaluates leukocyte infiltration, ICAM-1 expression, permeability across the blood-spinal cord barrier (BSCB)and therapeutic effect of ICAM-1 following epidural administration. Rabbits weighing ( 2.5 Kg) were anesthetized with halothane, an abdominal incision was made, the descending aorta exposed and occluded with an aneusysm clip. After varible periods of occlusion, the clip was removed and incision closed with sutures and skin clips. Sham-operated controls were prepared identically but descending aorta was not occluded. Following 2,4 and 8h after 30 min occlusion the following were evaluated in the lumbar cord: ICAM-1 expression by immunohistochemistry; leukocyte infiltration by electron microscopy; vascular endothelial permeability across BSCB by radiometry. The therapeutic efficacy of ICAM-1 MAb was tested by preemptive epidural administration of 0.5mg/Kg ICAM-1 MAb, following variable occlusion periods, i.e., 10,15.20,25 and 30 min. The neurological examination was performed 20h later on a Tarlov's scale in a blinded fashion. The ICAM-1 expression and leukocyte recruitment/infiltration were significantly increased following 8h after 30 min occlusion. The BSCB permeability of D-mannitol and inulin was significantly increased. The quantal response curve computed as dichotomous function of non-paraplegia/paraplegia Vs occlusion period based on neurologic evaluation, revealed that treatment with ICAM-1 MAb produced significant reduction in neurological deficits (P<0.05)in this model.
AB - This study evaluates leukocyte infiltration, ICAM-1 expression, permeability across the blood-spinal cord barrier (BSCB)and therapeutic effect of ICAM-1 following epidural administration. Rabbits weighing ( 2.5 Kg) were anesthetized with halothane, an abdominal incision was made, the descending aorta exposed and occluded with an aneusysm clip. After varible periods of occlusion, the clip was removed and incision closed with sutures and skin clips. Sham-operated controls were prepared identically but descending aorta was not occluded. Following 2,4 and 8h after 30 min occlusion the following were evaluated in the lumbar cord: ICAM-1 expression by immunohistochemistry; leukocyte infiltration by electron microscopy; vascular endothelial permeability across BSCB by radiometry. The therapeutic efficacy of ICAM-1 MAb was tested by preemptive epidural administration of 0.5mg/Kg ICAM-1 MAb, following variable occlusion periods, i.e., 10,15.20,25 and 30 min. The neurological examination was performed 20h later on a Tarlov's scale in a blinded fashion. The ICAM-1 expression and leukocyte recruitment/infiltration were significantly increased following 8h after 30 min occlusion. The BSCB permeability of D-mannitol and inulin was significantly increased. The quantal response curve computed as dichotomous function of non-paraplegia/paraplegia Vs occlusion period based on neurologic evaluation, revealed that treatment with ICAM-1 MAb produced significant reduction in neurological deficits (P<0.05)in this model.
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UR - http://www.scopus.com/inward/citedby.url?scp=33749320660&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:33749320660
VL - 12
JO - FASEB Journal
JF - FASEB Journal
SN - 0892-6638
IS - 5
ER -