Evidence for engraftment of human bone marrow cells in non-lethally irradiated baboons

Paulo Fontes, Jeffrey Rogers, Abdul S. Rao, Massimo Trucco, Adriana Zeevi, Camillo Ricordi, John J. Fung, Thomas E. Starzl

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Background. Prior to organ harvesting, an attempt was made to modulate the donor's immune responses against prospective xenogeneic recipients by infusion of 'recipient-type' bone marrow. Methods. For this purpose, baboons conditioned with total lymphoid irradiation were given 6x108 unmodified human bone marrow cells/kg body weight with no subsequent treatment. Results. Animals survived until they were euthanized at 18 months. Using primers specific for human chorionic gonadotrophin gene, the presence of human DNA was confirmed by polymerase chain reaction in the blood of one animal for up to 18 months after cell transplantation; in the other animal, xenogeneic chimerism became undetectable in the blood at 6 months after bone marrow infusion. However, tissue samples obtained from both animals at the time they were euthanized bad evidence of donor (human) DNA. Additionally, the presence of donor DNA in individually harvested colonies of erythroid and myeloid lineages suggested that infused human bone marrow cells had engrafted across the xenogeneic barrier in both baboons. Conclusions. Bone marrow transplantation from human to baboon leads to establishment of chimerism and modulation of donor-specific immune reactivity, which suggests that this strategy could be reproducibly employed to crease 'surrogate' tolerogenesis in prospective donors for subsequent organ transplantation across xenogeneic barriers.

Original languageEnglish (US)
Pages (from-to)1595-1698
Number of pages104
JournalTransplantation
Volume64
Issue number11
DOIs
StatePublished - Dec 15 1997

ASJC Scopus subject areas

  • Transplantation

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    Fontes, P., Rogers, J., Rao, A. S., Trucco, M., Zeevi, A., Ricordi, C., Fung, J. J., & Starzl, T. E. (1997). Evidence for engraftment of human bone marrow cells in non-lethally irradiated baboons. Transplantation, 64(11), 1595-1698. https://doi.org/10.1097/00007890-199712150-00016