Abstract
Serological data identify a single major histocompatibility complex (MHC) class I locus in cattle. Molecular data, however, demonstrate the presence of at least two cattle MHC (BoLA) class I loci. To investigate the number of transcribed BoLA class I genes, we amplified cattle cDNA by using a single MHC class I-specific primer that hybridized to a conserved region of exon 4 and a non-specific 3′ primer. Six BoLA class I cDNAs have been cloned and sequenced from a Bos taurus bull heterozygous for BoLA class I serological antigens, demonstrating the presence of a minimum of three loci. Sequence comparisons suggested that one of these cDNAs may be an unexpressed allele or the product of a nonclassical locus.
Original language | English (US) |
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Pages (from-to) | 257-265 |
Number of pages | 9 |
Journal | Immunogenetics |
Volume | 39 |
Issue number | 4 |
DOIs | |
State | Published - Feb 1994 |
Externally published | Yes |
ASJC Scopus subject areas
- Immunology
- Genetics