Evidence for at least three transcribed BoLA class I loci

Theodore L. Garber, Austin L. Hughes, David Watkins, Joe W. Templeton

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Serological data identify a single major histocompatibility complex (MHC) class I locus in cattle. Molecular data, however, demonstrate the presence of at least two cattle MHC (BoLA) class I loci. To investigate the number of transcribed BoLA class I genes, we amplified cattle cDNA by using a single MHC class I-specific primer that hybridized to a conserved region of exon 4 and a non-specific 3′ primer. Six BoLA class I cDNAs have been cloned and sequenced from a Bos taurus bull heterozygous for BoLA class I serological antigens, demonstrating the presence of a minimum of three loci. Sequence comparisons suggested that one of these cDNAs may be an unexpressed allele or the product of a nonclassical locus.

Original languageEnglish
Pages (from-to)257-265
Number of pages9
JournalImmunogenetics
Volume39
Issue number4
DOIs
StatePublished - Feb 1 1994
Externally publishedYes

Fingerprint

Major Histocompatibility Complex
Complementary DNA
MHC Class I Genes
Histocompatibility Antigens Class I
Exons
Alleles

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Evidence for at least three transcribed BoLA class I loci. / Garber, Theodore L.; Hughes, Austin L.; Watkins, David; Templeton, Joe W.

In: Immunogenetics, Vol. 39, No. 4, 01.02.1994, p. 257-265.

Research output: Contribution to journalArticle

Garber, TL, Hughes, AL, Watkins, D & Templeton, JW 1994, 'Evidence for at least three transcribed BoLA class I loci', Immunogenetics, vol. 39, no. 4, pp. 257-265. https://doi.org/10.1007/BF00188788
Garber, Theodore L. ; Hughes, Austin L. ; Watkins, David ; Templeton, Joe W. / Evidence for at least three transcribed BoLA class I loci. In: Immunogenetics. 1994 ; Vol. 39, No. 4. pp. 257-265.
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