Abstract
Serological data identify a single major histocompatibility complex (MHC) class I locus in cattle. Molecular data, however, demonstrate the presence of at least two cattle MHC (BoLA) class I loci. To investigate the number of transcribed BoLA class I genes, we amplified cattle cDNA by using a single MHC class I-specific primer that hybridized to a conserved region of exon 4 and a non-specific 3′ primer. Six BoLA class I cDNAs have been cloned and sequenced from a Bos taurus bull heterozygous for BoLA class I serological antigens, demonstrating the presence of a minimum of three loci. Sequence comparisons suggested that one of these cDNAs may be an unexpressed allele or the product of a nonclassical locus.
| Original language | English |
|---|---|
| Pages (from-to) | 257-265 |
| Number of pages | 9 |
| Journal | Immunogenetics |
| Volume | 39 |
| Issue number | 4 |
| DOIs | |
| State | Published - Feb 1 1994 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
- Genetics
Cite this
Evidence for at least three transcribed BoLA class I loci. / Garber, Theodore L.; Hughes, Austin L.; Watkins, David; Templeton, Joe W.
In: Immunogenetics, Vol. 39, No. 4, 01.02.1994, p. 257-265.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evidence for at least three transcribed BoLA class I loci
AU - Garber, Theodore L.
AU - Hughes, Austin L.
AU - Watkins, David
AU - Templeton, Joe W.
PY - 1994/2/1
Y1 - 1994/2/1
N2 - Serological data identify a single major histocompatibility complex (MHC) class I locus in cattle. Molecular data, however, demonstrate the presence of at least two cattle MHC (BoLA) class I loci. To investigate the number of transcribed BoLA class I genes, we amplified cattle cDNA by using a single MHC class I-specific primer that hybridized to a conserved region of exon 4 and a non-specific 3′ primer. Six BoLA class I cDNAs have been cloned and sequenced from a Bos taurus bull heterozygous for BoLA class I serological antigens, demonstrating the presence of a minimum of three loci. Sequence comparisons suggested that one of these cDNAs may be an unexpressed allele or the product of a nonclassical locus.
AB - Serological data identify a single major histocompatibility complex (MHC) class I locus in cattle. Molecular data, however, demonstrate the presence of at least two cattle MHC (BoLA) class I loci. To investigate the number of transcribed BoLA class I genes, we amplified cattle cDNA by using a single MHC class I-specific primer that hybridized to a conserved region of exon 4 and a non-specific 3′ primer. Six BoLA class I cDNAs have been cloned and sequenced from a Bos taurus bull heterozygous for BoLA class I serological antigens, demonstrating the presence of a minimum of three loci. Sequence comparisons suggested that one of these cDNAs may be an unexpressed allele or the product of a nonclassical locus.
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U2 - 10.1007/BF00188788
DO - 10.1007/BF00188788
M3 - Article
VL - 39
SP - 257
EP - 265
JO - Immunogenetics
JF - Immunogenetics
SN - 0093-7711
IS - 4
ER -