Evidence for at least three transcribed BoLA class I loci

Theodore L. Garber, Austin L. Hughes, David I. Watkins, Joe W. Templeton

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Serological data identify a single major histocompatibility complex (MHC) class I locus in cattle. Molecular data, however, demonstrate the presence of at least two cattle MHC (BoLA) class I loci. To investigate the number of transcribed BoLA class I genes, we amplified cattle cDNA by using a single MHC class I-specific primer that hybridized to a conserved region of exon 4 and a non-specific 3′ primer. Six BoLA class I cDNAs have been cloned and sequenced from a Bos taurus bull heterozygous for BoLA class I serological antigens, demonstrating the presence of a minimum of three loci. Sequence comparisons suggested that one of these cDNAs may be an unexpressed allele or the product of a nonclassical locus.

Original languageEnglish (US)
Pages (from-to)257-265
Number of pages9
JournalImmunogenetics
Volume39
Issue number4
DOIs
StatePublished - Feb 1994
Externally publishedYes

ASJC Scopus subject areas

  • Immunology
  • Genetics

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