Evidence for a pro-calcitonin

B. A. Roos, K. Okano, L. J. Deftos

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The biosynthesis of calcitonin was studied using radioimmunochemical methods and suspensions of calcitonin-producing cells derived from trout ultimobranchial glands. [14C]leucine was incorporated into cell proteins in a linear fashion for up to 36 hrs. Acid-extracted cellular radioactivity could be precipitated by trichloroacetic acid and calcitonin antiserum. Chromatography of the cell extracts revealed two distinct peaks of radio-immunoassayable and immunoprecipitable calcitonin activity. One peak coeluted with radioiodinated calcitonin, the other as a higher molecular weight species. The relative incorporation of [14C]leucine into the higher and lower molecular weight peaks during "pulse-chase" experiments was consistent with a precursor-product relationship between them.

Original languageEnglish
Pages (from-to)1134-1140
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume60
Issue number3
DOIs
StatePublished - Oct 8 1974
Externally publishedYes

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Calcitonin
Leucine
Molecular Weight
Molecular weight
Trichloroacetic Acid
Trout
Biosynthesis
Radioactivity
Chromatography
Cell Extracts
Radio
Immune Sera
Suspensions
Acids
Proteins
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Evidence for a pro-calcitonin. / Roos, B. A.; Okano, K.; Deftos, L. J.

In: Biochemical and Biophysical Research Communications, Vol. 60, No. 3, 08.10.1974, p. 1134-1140.

Research output: Contribution to journalArticle

Roos, B. A. ; Okano, K. ; Deftos, L. J. / Evidence for a pro-calcitonin. In: Biochemical and Biophysical Research Communications. 1974 ; Vol. 60, No. 3. pp. 1134-1140.
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