Evaluation of the usefulness of an ELISA and protein electrophoresis in the diagnosis of Encephalitozoon cuniculi infection in rabbits

Carolyn Cray, Giselle Arcia, Renata Schneider, Susan A. Kelleher, Kristopher Arheart

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Objective - To evaluate the usefulness of an antibody detection ELISA and protein electrophoresis (PE) for diagnosing Encephalitozoon cuniculi (ECUN) infection in pet rabbits. Animals - 203 pet rabbits. Procedures - Serum and plasma samples from pet rabbits were submitted from veterinary clinics within the United States. Participating veterinarians completed a questionnaire that was used to classify rabbits as clinically normal (n=33), suspected of having an ECUN infection (103), or clinically abnormal but not suspected of having an ECUN infection (67). An ELISA for detection of serum or plasma IgG against ECUN was developed by use of commercially available reagents. Results of the ELISA and PE were used to detect ECUN infection. Results - A high seroprevalence of antibody against ECUN was detected in all 3 groups of rabbits. In rabbits suspected of having an ECUN infection, the mean IgG titer was 1.7 times as high as the values in the other rabbit groups. Rabbits suspected of having an ECUN infection and those that were simply clinically abnormal had a higher concentration of γ-globulins than clinically normal rabbits. This increase in globulins concentration was accompanied by a decrease in the albumin-to-globulin ratio. Results of the ELISA and PE were significantly different between clinically normal rabbits and those suspected of having an ECUN infection. Conclusions and Clinical Relevance - The combination of an ELISA and PE may aid in the diagnosis of ECUN infection in pet rabbits. Impact for Human Medidine - Because ECUN is a potential zoonotic agent, diagnostic methods for pet rabbits need to be improved to protect human health.

Original languageEnglish
Pages (from-to)478-482
Number of pages5
JournalAmerican Journal of Veterinary Research
Volume70
Issue number4
DOIs
StatePublished - Apr 1 2009

Fingerprint

Encephalitozoon cuniculi
electrophoresis
Electrophoresis
Enzyme-Linked Immunosorbent Assay
rabbits
enzyme-linked immunosorbent assay
Rabbits
Infection
infection
Pets
Proteins
proteins
pets
Globulins
globulins
Immunoglobulin G
Animal Hospitals
Veterinarians
Antibodies
veterinary clinics

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Evaluation of the usefulness of an ELISA and protein electrophoresis in the diagnosis of Encephalitozoon cuniculi infection in rabbits. / Cray, Carolyn; Arcia, Giselle; Schneider, Renata; Kelleher, Susan A.; Arheart, Kristopher.

In: American Journal of Veterinary Research, Vol. 70, No. 4, 01.04.2009, p. 478-482.

Research output: Contribution to journalArticle

Cray, Carolyn ; Arcia, Giselle ; Schneider, Renata ; Kelleher, Susan A. ; Arheart, Kristopher. / Evaluation of the usefulness of an ELISA and protein electrophoresis in the diagnosis of Encephalitozoon cuniculi infection in rabbits. In: American Journal of Veterinary Research. 2009 ; Vol. 70, No. 4. pp. 478-482.
@article{48b4c1e255b645a5ad39bd4cd3bce6d1,
title = "Evaluation of the usefulness of an ELISA and protein electrophoresis in the diagnosis of Encephalitozoon cuniculi infection in rabbits",
abstract = "Objective - To evaluate the usefulness of an antibody detection ELISA and protein electrophoresis (PE) for diagnosing Encephalitozoon cuniculi (ECUN) infection in pet rabbits. Animals - 203 pet rabbits. Procedures - Serum and plasma samples from pet rabbits were submitted from veterinary clinics within the United States. Participating veterinarians completed a questionnaire that was used to classify rabbits as clinically normal (n=33), suspected of having an ECUN infection (103), or clinically abnormal but not suspected of having an ECUN infection (67). An ELISA for detection of serum or plasma IgG against ECUN was developed by use of commercially available reagents. Results of the ELISA and PE were used to detect ECUN infection. Results - A high seroprevalence of antibody against ECUN was detected in all 3 groups of rabbits. In rabbits suspected of having an ECUN infection, the mean IgG titer was 1.7 times as high as the values in the other rabbit groups. Rabbits suspected of having an ECUN infection and those that were simply clinically abnormal had a higher concentration of γ-globulins than clinically normal rabbits. This increase in globulins concentration was accompanied by a decrease in the albumin-to-globulin ratio. Results of the ELISA and PE were significantly different between clinically normal rabbits and those suspected of having an ECUN infection. Conclusions and Clinical Relevance - The combination of an ELISA and PE may aid in the diagnosis of ECUN infection in pet rabbits. Impact for Human Medidine - Because ECUN is a potential zoonotic agent, diagnostic methods for pet rabbits need to be improved to protect human health.",
author = "Carolyn Cray and Giselle Arcia and Renata Schneider and Kelleher, {Susan A.} and Kristopher Arheart",
year = "2009",
month = "4",
day = "1",
doi = "10.2460/ajvr.70.4.478",
language = "English",
volume = "70",
pages = "478--482",
journal = "American Journal of Veterinary Research",
issn = "0002-9645",
publisher = "American Veterinary Medical Association",
number = "4",

}

TY - JOUR

T1 - Evaluation of the usefulness of an ELISA and protein electrophoresis in the diagnosis of Encephalitozoon cuniculi infection in rabbits

AU - Cray, Carolyn

AU - Arcia, Giselle

AU - Schneider, Renata

AU - Kelleher, Susan A.

AU - Arheart, Kristopher

PY - 2009/4/1

Y1 - 2009/4/1

N2 - Objective - To evaluate the usefulness of an antibody detection ELISA and protein electrophoresis (PE) for diagnosing Encephalitozoon cuniculi (ECUN) infection in pet rabbits. Animals - 203 pet rabbits. Procedures - Serum and plasma samples from pet rabbits were submitted from veterinary clinics within the United States. Participating veterinarians completed a questionnaire that was used to classify rabbits as clinically normal (n=33), suspected of having an ECUN infection (103), or clinically abnormal but not suspected of having an ECUN infection (67). An ELISA for detection of serum or plasma IgG against ECUN was developed by use of commercially available reagents. Results of the ELISA and PE were used to detect ECUN infection. Results - A high seroprevalence of antibody against ECUN was detected in all 3 groups of rabbits. In rabbits suspected of having an ECUN infection, the mean IgG titer was 1.7 times as high as the values in the other rabbit groups. Rabbits suspected of having an ECUN infection and those that were simply clinically abnormal had a higher concentration of γ-globulins than clinically normal rabbits. This increase in globulins concentration was accompanied by a decrease in the albumin-to-globulin ratio. Results of the ELISA and PE were significantly different between clinically normal rabbits and those suspected of having an ECUN infection. Conclusions and Clinical Relevance - The combination of an ELISA and PE may aid in the diagnosis of ECUN infection in pet rabbits. Impact for Human Medidine - Because ECUN is a potential zoonotic agent, diagnostic methods for pet rabbits need to be improved to protect human health.

AB - Objective - To evaluate the usefulness of an antibody detection ELISA and protein electrophoresis (PE) for diagnosing Encephalitozoon cuniculi (ECUN) infection in pet rabbits. Animals - 203 pet rabbits. Procedures - Serum and plasma samples from pet rabbits were submitted from veterinary clinics within the United States. Participating veterinarians completed a questionnaire that was used to classify rabbits as clinically normal (n=33), suspected of having an ECUN infection (103), or clinically abnormal but not suspected of having an ECUN infection (67). An ELISA for detection of serum or plasma IgG against ECUN was developed by use of commercially available reagents. Results of the ELISA and PE were used to detect ECUN infection. Results - A high seroprevalence of antibody against ECUN was detected in all 3 groups of rabbits. In rabbits suspected of having an ECUN infection, the mean IgG titer was 1.7 times as high as the values in the other rabbit groups. Rabbits suspected of having an ECUN infection and those that were simply clinically abnormal had a higher concentration of γ-globulins than clinically normal rabbits. This increase in globulins concentration was accompanied by a decrease in the albumin-to-globulin ratio. Results of the ELISA and PE were significantly different between clinically normal rabbits and those suspected of having an ECUN infection. Conclusions and Clinical Relevance - The combination of an ELISA and PE may aid in the diagnosis of ECUN infection in pet rabbits. Impact for Human Medidine - Because ECUN is a potential zoonotic agent, diagnostic methods for pet rabbits need to be improved to protect human health.

UR - http://www.scopus.com/inward/record.url?scp=65349171897&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65349171897&partnerID=8YFLogxK

U2 - 10.2460/ajvr.70.4.478

DO - 10.2460/ajvr.70.4.478

M3 - Article

VL - 70

SP - 478

EP - 482

JO - American Journal of Veterinary Research

JF - American Journal of Veterinary Research

SN - 0002-9645

IS - 4

ER -