Evaluation of the mitochondrial respiratory Chain and oxidative phosphorylation system using polarography and spectrophotometric enzyme assays

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Abstract

The oxidative phosphorylation (OXPHOS) system consists of five multimeric complexes embedded in the mitochondrial innermembrane. Theywork in concert to drive the aerobic synthesis of ATP. Mitochondrial and nuclear DNA mutations affecting the accumulation and function of these enzymes are the most common cause of mitochondrial diseases and have also been associated with neurodegeneration and aging. For this reason, several approaches for the assessment of the OXPHOS system enzymes have been developed. Based on the methods described elsewhere, the assays describe methods that form a biochemical characterization of the OXPHOS system in cells and mitochondria isolated from cultured cells or tissues. Curr. Protoc. Hum. Genet. 63:19.3.1-19.3.14.

Original languageEnglish
JournalCurrent Protocols in Human Genetics
Issue numberSUPPL.63
DOIs
StatePublished - Dec 1 2009

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Polarography
Oxidative Phosphorylation
Enzyme Assays
Electron Transport
Viverridae
Mitochondrial Diseases
Enzymes
Mitochondrial DNA
Cultured Cells
Mitochondria
Adenosine Triphosphate

Keywords

  • Electron transport chain
  • Mitochondria
  • OXPHOS
  • Polarography
  • Spectrophotometry

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

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