Purpose. To assess the biocolonization of porous membranes for keratoprosthesis grafted with biologically active molecules. Methods. Three different porous e-PTFE membranes were implanted in twelve rabbits. Type 1: 130pm thick, 30-60u,m pores, 94% porosity; Type 2: 130p:m thick, lOm pores, 75% porosity; Type 3 (control): 50-80(im pores, 260u;m thick, 85% porosity. One surface of one-half of the type 1 and type 2 membranes was modified with a covalent attachment of proteins designed to promote cell and fibrosis colonization. Two groups of six rabbits were unilaterally implanted with three polymers: Group 1: Type I treated and non-treated, and type 3; Group 2: Type 2 treated and non-treated, and type 3. The animals were followed-up for a maximum period of 180 days and euthanized at 7, 14, 28, 80 and 180 postoperative days for histology. Results. The acute inflammatory response was more important with the treated polymers, with an important neovascularization coming from the limbus to the polymers by the anterior side. This inflammation ended after two weeks with a stabilized anterior new vascularisation localized on the anterior corneal stroma. Extrusion of the polymer was observed in three cases. The biocolonization of the treated and non-treated polymers was significantly different as far as the cell type and density, and fibrosis response. Conclusions. Surface modification resulted in better biocolonization and seems promising for keratoprosthesis anchoring. Further investigation is necessary to assess the long term effect of neovascularization.
|Original language||English (US)|
|Journal||Investigative Ophthalmology and Visual Science|
|State||Published - Dec 1 1997|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience