Evaluation of Methods to Improve the Diagnosis of Systemic Inflammation in Alpacas

T. Passler, M. F. Chamorro, K. P. Riddell, M. A. Edmondson, E. van Santen, Carolyn Cray, H. S. Maxwell, P. H. Walz

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: The stoic nature of alpacas and limitations of current diagnostic tests make early recognition of inflammatory diseases in this species challenging. Objectives: In a model of mild systemic inflammation, this study evaluated the utility of different clinical and clinicopathologic variables as accurate predictors of inflammation in alpacas. Animals: Twelve clinically healthy alpacas were randomly assigned to equal-sized treatment (TG) and control (CG) groups. After collection of initial blood samples (0 hour), lipopolysaccharide (LPS; 20 μg/kg/24 h) or saline was administered by SC osmotic mini-pumps (OMP) for 96 hours. Additional blood samples were collected at 12, 18, 24, 36, 48, 72, 96, 120, 144, and 240 hours and differential leukocyte counts and concentrations of globulin, albumin, iron, haptoglobin, and serum amyloid A were measured. Results: Mild swelling was observed at OMP implantation sites in both groups. Other clinical signs of systemic inflammation were not observed. Total leukocytes, neutrophils, albumin, and globulin concentrations were not significantly different between groups. Compared with CG-alpacas, TG-alpacas had fewer lymphocytes (P = .0322), more band neutrophils (P = .0087), and higher neutrophil/lymphocyte ratios (P = .0295) during the first 96 hours of the study. During LPS administration, serum iron concentrations were significantly decreased in TG-alpacas (P < .0001). Haptoglobin concentrations of TG-animals exceeded those of CG-animals after removal of OMP (P = .0056). Serum amyloid A was not detectable in alpacas in this study. Conclusion and Clinical Importance: These results indicated that neutrophil/lymphocyte ratios and serum iron concentrations are early indicators of inflammation in alpacas. Additional research is needed to evaluate the acute phase protein responses of alpacas.

Original languageEnglish
Pages (from-to)970-976
Number of pages7
JournalJournal of Veterinary Internal Medicine
Volume27
Issue number4
DOIs
StatePublished - Jul 1 2013

Fingerprint

New World Camelids
alpacas
inflammation
Inflammation
blood serum
neutrophils
Neutrophils
pumps
Serum Amyloid A Protein
Haptoglobins
lymphocytes
Iron
haptoglobins
methodology
Globulins
Lymphocytes
amyloid
iron
globulins
albumins

Keywords

  • Acute phase proteins
  • Haptoglobin
  • New World camelid
  • Osmotic mini-pump
  • Serum amyloid A
  • Serum iron concentration

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Passler, T., Chamorro, M. F., Riddell, K. P., Edmondson, M. A., van Santen, E., Cray, C., ... Walz, P. H. (2013). Evaluation of Methods to Improve the Diagnosis of Systemic Inflammation in Alpacas. Journal of Veterinary Internal Medicine, 27(4), 970-976. https://doi.org/10.1111/jvim.12102

Evaluation of Methods to Improve the Diagnosis of Systemic Inflammation in Alpacas. / Passler, T.; Chamorro, M. F.; Riddell, K. P.; Edmondson, M. A.; van Santen, E.; Cray, Carolyn; Maxwell, H. S.; Walz, P. H.

In: Journal of Veterinary Internal Medicine, Vol. 27, No. 4, 01.07.2013, p. 970-976.

Research output: Contribution to journalArticle

Passler, T, Chamorro, MF, Riddell, KP, Edmondson, MA, van Santen, E, Cray, C, Maxwell, HS & Walz, PH 2013, 'Evaluation of Methods to Improve the Diagnosis of Systemic Inflammation in Alpacas', Journal of Veterinary Internal Medicine, vol. 27, no. 4, pp. 970-976. https://doi.org/10.1111/jvim.12102
Passler, T. ; Chamorro, M. F. ; Riddell, K. P. ; Edmondson, M. A. ; van Santen, E. ; Cray, Carolyn ; Maxwell, H. S. ; Walz, P. H. / Evaluation of Methods to Improve the Diagnosis of Systemic Inflammation in Alpacas. In: Journal of Veterinary Internal Medicine. 2013 ; Vol. 27, No. 4. pp. 970-976.
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