Evaluation of innate immune signaling pathways in transformed cells

Joshua F. Heiber, Glen N Barber

Research output: Chapter in Book/Report/Conference proceedingChapter

8 Citations (Scopus)

Abstract

Oncolytic viruses, the use of viruses to treat cancer, is emerging as a new option for cancer therapy. Oncolytic viruses, of both DNA and RNA origin, exhibit the ability to preferentially replicate in and kill cancer cells plausibly due to defects in innate immune signaling or translation regulation that are acquired during cellular transformation. Here, we review concepts and assays that describe how to analyze signaling pathways that govern the regulation of Type I IFN production as well as the induction of interferon-stimulated antiviral genes, events that are critical for mounting an effective antiviral response. The following procedures can be used to assess whether innate immune pathways that control antiviral host defense are defective in tumor cells-mechanisms that may help to explain viral oncolysis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages217-238
Number of pages22
Volume797
DOIs
StatePublished - Jan 1 2012

Publication series

NameMethods in Molecular Biology
Volume797
ISSN (Print)10643745

Fingerprint

Oncolytic Viruses
Antiviral Agents
Neoplasms
Interferons
RNA
Viruses
DNA
Genes
Therapeutics

Keywords

  • Host defense
  • Innate immunity
  • Interferon
  • Oncolytic
  • STING

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Heiber, J. F., & Barber, G. N. (2012). Evaluation of innate immune signaling pathways in transformed cells. In Methods in Molecular Biology (Vol. 797, pp. 217-238). (Methods in Molecular Biology; Vol. 797). https://doi.org/10.1007/978-1-61779-340-0_15

Evaluation of innate immune signaling pathways in transformed cells. / Heiber, Joshua F.; Barber, Glen N.

Methods in Molecular Biology. Vol. 797 2012. p. 217-238 (Methods in Molecular Biology; Vol. 797).

Research output: Chapter in Book/Report/Conference proceedingChapter

Heiber, JF & Barber, GN 2012, Evaluation of innate immune signaling pathways in transformed cells. in Methods in Molecular Biology. vol. 797, Methods in Molecular Biology, vol. 797, pp. 217-238. https://doi.org/10.1007/978-1-61779-340-0_15
Heiber JF, Barber GN. Evaluation of innate immune signaling pathways in transformed cells. In Methods in Molecular Biology. Vol. 797. 2012. p. 217-238. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-340-0_15
Heiber, Joshua F. ; Barber, Glen N. / Evaluation of innate immune signaling pathways in transformed cells. Methods in Molecular Biology. Vol. 797 2012. pp. 217-238 (Methods in Molecular Biology).
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