Abstract
Oncolytic viruses, the use of viruses to treat cancer, is emerging as a new option for cancer therapy. Oncolytic viruses, of both DNA and RNA origin, exhibit the ability to preferentially replicate in and kill cancer cells plausibly due to defects in innate immune signaling or translation regulation that are acquired during cellular transformation. Here, we review concepts and assays that describe how to analyze signaling pathways that govern the regulation of Type I IFN production as well as the induction of interferon-stimulated antiviral genes, events that are critical for mounting an effective antiviral response. The following procedures can be used to assess whether innate immune pathways that control antiviral host defense are defective in tumor cells-mechanisms that may help to explain viral oncolysis.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Pages | 217-238 |
| Number of pages | 22 |
| Volume | 797 |
| DOIs | |
| State | Published - Jan 1 2012 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 797 |
| ISSN (Print) | 10643745 |
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Keywords
- Host defense
- Innate immunity
- Interferon
- Oncolytic
- STING
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Evaluation of innate immune signaling pathways in transformed cells. / Heiber, Joshua F.; Barber, Glen N.
Methods in Molecular Biology. Vol. 797 2012. p. 217-238 (Methods in Molecular Biology; Vol. 797).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Evaluation of innate immune signaling pathways in transformed cells
AU - Heiber, Joshua F.
AU - Barber, Glen N
PY - 2012/1/1
Y1 - 2012/1/1
N2 - Oncolytic viruses, the use of viruses to treat cancer, is emerging as a new option for cancer therapy. Oncolytic viruses, of both DNA and RNA origin, exhibit the ability to preferentially replicate in and kill cancer cells plausibly due to defects in innate immune signaling or translation regulation that are acquired during cellular transformation. Here, we review concepts and assays that describe how to analyze signaling pathways that govern the regulation of Type I IFN production as well as the induction of interferon-stimulated antiviral genes, events that are critical for mounting an effective antiviral response. The following procedures can be used to assess whether innate immune pathways that control antiviral host defense are defective in tumor cells-mechanisms that may help to explain viral oncolysis.
AB - Oncolytic viruses, the use of viruses to treat cancer, is emerging as a new option for cancer therapy. Oncolytic viruses, of both DNA and RNA origin, exhibit the ability to preferentially replicate in and kill cancer cells plausibly due to defects in innate immune signaling or translation regulation that are acquired during cellular transformation. Here, we review concepts and assays that describe how to analyze signaling pathways that govern the regulation of Type I IFN production as well as the induction of interferon-stimulated antiviral genes, events that are critical for mounting an effective antiviral response. The following procedures can be used to assess whether innate immune pathways that control antiviral host defense are defective in tumor cells-mechanisms that may help to explain viral oncolysis.
KW - Host defense
KW - Innate immunity
KW - Interferon
KW - Oncolytic
KW - STING
UR - http://www.scopus.com/inward/record.url?scp=80555127439&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80555127439&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-340-0_15
DO - 10.1007/978-1-61779-340-0_15
M3 - Chapter
SN - 9781617793394
VL - 797
T3 - Methods in Molecular Biology
SP - 217
EP - 238
BT - Methods in Molecular Biology
ER -