Abstract
Accumulation of potassium and excitatory amino acids (EAA) in the extracellular space (ECS) following ischemia has been well documented. Careful monitoring of these transients is crucial to gain a better understanding of CNS pathophysiology. This study was initiated to determine if CSF concentrations of EAAs reflect those measured in the ECS. Transient global ischemia, 20 minutes in duration, was produced by clamping the left subclavian and innominate arteries combined with hemorrhagic hypotension. The accumulation of glutamate and electrolytes were measured in CSF and the extracellular fluid (ECF) of cerebral cortex. Microdialysis (MD) was utilized to measure the extracellular concentrations while direct sampling of CSF was provided via cannulation of the cisterna magna. Hydrogen clearance and laser doppler methods were used to monitor regional cortical CBF. Our results show that extracellular concentrations of potassium ([K+]ECF) and glutamate significantly increased following the initiation of ischemia (p < 0.05). The extracellular concentration of these substances decreased with the restoration of CBF. In CSF, a similar trend was observed following re-circulation (p < 0.05). However, CSF glutamate levels did not return to pre-ischemic values.
| Original language | English |
|---|---|
| Pages (from-to) | 71-74 |
| Number of pages | 4 |
| Journal | Acta Neurochirurgica, Supplement |
| Volume | 1997 |
| Issue number | 70 |
| State | Published - Dec 1 1997 |
| Externally published | Yes |
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Keywords
- Cerebral ischemia
- Cerebrospinal fluid
- Extracellular glutamate
- Homeoslatic changes
ASJC Scopus subject areas
- Medicine(all)
- Clinical Neurology
Cite this
Evaluation of Homeostatic Changes in CSF Circulation : In vivo Analysis of the Effect of Neurotransmitter Accumulation in the Extracellular Space Following Transient Global Ischemia. / Yamamoto, T.; Marmarou, A.; Stiefel, M. F.; Tsuji, O.; Bullock, Ross.
In: Acta Neurochirurgica, Supplement, Vol. 1997, No. 70, 01.12.1997, p. 71-74.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of Homeostatic Changes in CSF Circulation
T2 - In vivo Analysis of the Effect of Neurotransmitter Accumulation in the Extracellular Space Following Transient Global Ischemia
AU - Yamamoto, T.
AU - Marmarou, A.
AU - Stiefel, M. F.
AU - Tsuji, O.
AU - Bullock, Ross
PY - 1997/12/1
Y1 - 1997/12/1
N2 - Accumulation of potassium and excitatory amino acids (EAA) in the extracellular space (ECS) following ischemia has been well documented. Careful monitoring of these transients is crucial to gain a better understanding of CNS pathophysiology. This study was initiated to determine if CSF concentrations of EAAs reflect those measured in the ECS. Transient global ischemia, 20 minutes in duration, was produced by clamping the left subclavian and innominate arteries combined with hemorrhagic hypotension. The accumulation of glutamate and electrolytes were measured in CSF and the extracellular fluid (ECF) of cerebral cortex. Microdialysis (MD) was utilized to measure the extracellular concentrations while direct sampling of CSF was provided via cannulation of the cisterna magna. Hydrogen clearance and laser doppler methods were used to monitor regional cortical CBF. Our results show that extracellular concentrations of potassium ([K+]ECF) and glutamate significantly increased following the initiation of ischemia (p < 0.05). The extracellular concentration of these substances decreased with the restoration of CBF. In CSF, a similar trend was observed following re-circulation (p < 0.05). However, CSF glutamate levels did not return to pre-ischemic values.
AB - Accumulation of potassium and excitatory amino acids (EAA) in the extracellular space (ECS) following ischemia has been well documented. Careful monitoring of these transients is crucial to gain a better understanding of CNS pathophysiology. This study was initiated to determine if CSF concentrations of EAAs reflect those measured in the ECS. Transient global ischemia, 20 minutes in duration, was produced by clamping the left subclavian and innominate arteries combined with hemorrhagic hypotension. The accumulation of glutamate and electrolytes were measured in CSF and the extracellular fluid (ECF) of cerebral cortex. Microdialysis (MD) was utilized to measure the extracellular concentrations while direct sampling of CSF was provided via cannulation of the cisterna magna. Hydrogen clearance and laser doppler methods were used to monitor regional cortical CBF. Our results show that extracellular concentrations of potassium ([K+]ECF) and glutamate significantly increased following the initiation of ischemia (p < 0.05). The extracellular concentration of these substances decreased with the restoration of CBF. In CSF, a similar trend was observed following re-circulation (p < 0.05). However, CSF glutamate levels did not return to pre-ischemic values.
KW - Cerebral ischemia
KW - Cerebrospinal fluid
KW - Extracellular glutamate
KW - Homeoslatic changes
UR - http://www.scopus.com/inward/record.url?scp=0031310611&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031310611&partnerID=8YFLogxK
M3 - Article
C2 - 9416282
AN - SCOPUS:0031310611
VL - 1997
SP - 71
EP - 74
JO - Scientific Computing and Instrumentation
JF - Scientific Computing and Instrumentation
SN - 1078-8956
IS - 70
ER -