Evaluation of Homeostatic Changes in CSF Circulation

In vivo Analysis of the Effect of Neurotransmitter Accumulation in the Extracellular Space Following Transient Global Ischemia

T. Yamamoto, A. Marmarou, M. F. Stiefel, O. Tsuji, Ross Bullock

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Accumulation of potassium and excitatory amino acids (EAA) in the extracellular space (ECS) following ischemia has been well documented. Careful monitoring of these transients is crucial to gain a better understanding of CNS pathophysiology. This study was initiated to determine if CSF concentrations of EAAs reflect those measured in the ECS. Transient global ischemia, 20 minutes in duration, was produced by clamping the left subclavian and innominate arteries combined with hemorrhagic hypotension. The accumulation of glutamate and electrolytes were measured in CSF and the extracellular fluid (ECF) of cerebral cortex. Microdialysis (MD) was utilized to measure the extracellular concentrations while direct sampling of CSF was provided via cannulation of the cisterna magna. Hydrogen clearance and laser doppler methods were used to monitor regional cortical CBF. Our results show that extracellular concentrations of potassium ([K+]ECF) and glutamate significantly increased following the initiation of ischemia (p < 0.05). The extracellular concentration of these substances decreased with the restoration of CBF. In CSF, a similar trend was observed following re-circulation (p < 0.05). However, CSF glutamate levels did not return to pre-ischemic values.

Original languageEnglish
Pages (from-to)71-74
Number of pages4
JournalActa Neurochirurgica, Supplement
Volume1997
Issue number70
StatePublished - Dec 1 1997
Externally publishedYes

Fingerprint

neurotransmitters
ischemia
Extracellular Space
glutamates
Neurotransmitter Agents
Potassium
Glutamic Acid
Ischemia
Extracellular Fluid
Fluids
evaluation
Brachiocephalic Trunk
Cisterna Magna
Restoration
Amino acids
potassium
hypotension
Excitatory Amino Acids
Subclavian Artery
Electrolytes

Keywords

  • Cerebral ischemia
  • Cerebrospinal fluid
  • Extracellular glutamate
  • Homeoslatic changes

ASJC Scopus subject areas

  • Medicine(all)
  • Clinical Neurology

Cite this

Evaluation of Homeostatic Changes in CSF Circulation : In vivo Analysis of the Effect of Neurotransmitter Accumulation in the Extracellular Space Following Transient Global Ischemia. / Yamamoto, T.; Marmarou, A.; Stiefel, M. F.; Tsuji, O.; Bullock, Ross.

In: Acta Neurochirurgica, Supplement, Vol. 1997, No. 70, 01.12.1997, p. 71-74.

Research output: Contribution to journalArticle

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