Objective: We present a novel method for screening eye bank donor corneas using high deﬁnition optical coherence tomography (HD-OCT). This technology allows for the quantiﬁcation of endothelial/Descemet membrane (En/DM) complex thickness ex vivo. Design: Prospective interventional study. Participants: Fifty-two corneal grafts from 27 donors were included in this study. Twenty additional control eyes and 11 eyes with Fuchs’ endothelial corneal dystrophy were also evaluated for comparison. Methods: A custom built, high speed HD-OCT device (Envisu R2210, Bioptigen, Buffalo Grove, IL, USA) was used to obtain images, and custom-made graph-based segmentation software was used to automatically deconstruct corneal images into micro-layers. HD-OCT imaging was used to scan through the sealed sterile case of donor corneas stored in McCarey-Kaufman medium to image their En/DM complex through the center of the cornea. Results: This technology allowed for quantiﬁcation of En/DM complex thickness in all donor corneas through the sealed sterile container used to transport graft tissue. Mean En/ DM complex thickness of donor corneas was 17±4 μm. The difference between donor cornea En/DM thickness and that of control subjects (16±2 μm) was not statistically signiﬁcant (p=0.3), suggesting that the transport container and media do not affect measurements. There was a signiﬁcant difference between En/DM thickness of Fuchs’ endothelial corneal dystrophy eyes (25±5 μm) and both donor corneas (p<0.0001) and control subjects (p<0.0001). Conclusions: We have described a new technique to measure En/DM complex thickness in eye bank donor corneas stored in a sealed sterile case. This may represent a novel adjunctive approach to screen corneal grafts for early endothelial disease.
- Corneal graft screening
- Corneal transplant
- Fuchs’ endothelial corneal dystrophy
- Microscope-integrated OCT
ASJC Scopus subject areas