Abstract
Objective: We present a novel method for screening eye bank donor corneas using high definition optical coherence tomography (HD-OCT). This technology allows for the quantification of endothelial/Descemet membrane (En/DM) complex thickness ex vivo. Design: Prospective interventional study. Participants: Fifty-two corneal grafts from 27 donors were included in this study. Twenty additional control eyes and 11 eyes with Fuchs’ endothelial corneal dystrophy were also evaluated for comparison. Methods: A custom built, high speed HD-OCT device (Envisu R2210, Bioptigen, Buffalo Grove, IL, USA) was used to obtain images, and custom-made graph-based segmentation software was used to automatically deconstruct corneal images into micro-layers. HD-OCT imaging was used to scan through the sealed sterile case of donor corneas stored in McCarey-Kaufman medium to image their En/DM complex through the center of the cornea. Results: This technology allowed for quantification of En/DM complex thickness in all donor corneas through the sealed sterile container used to transport graft tissue. Mean En/ DM complex thickness of donor corneas was 17±4 μm. The difference between donor cornea En/DM thickness and that of control subjects (16±2 μm) was not statistically significant (p=0.3), suggesting that the transport container and media do not affect measurements. There was a significant difference between En/DM thickness of Fuchs’ endothelial corneal dystrophy eyes (25±5 μm) and both donor corneas (p<0.0001) and control subjects (p<0.0001). Conclusions: We have described a new technique to measure En/DM complex thickness in eye bank donor corneas stored in a sealed sterile case. This may represent a novel adjunctive approach to screen corneal grafts for early endothelial disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 781-787 |
| Number of pages | 7 |
| Journal | Clinical Ophthalmology |
| Volume | 13 |
| DOIs | |
| State | Published - Jan 1 2019 |
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Keywords
- Corneal graft screening
- Corneal transplant
- Fuchs’ endothelial corneal dystrophy
- Microscope-integrated OCT
ASJC Scopus subject areas
- Ophthalmology
Cite this
Evaluation of endothelial/Descemet membrane complex of eye bank donor corneas using enhanced depth imaging optical coherence tomography. / Syed, Zeba A.; Gameiro, Gustavo Rosa; Ruggeri, Marco; Elsawy, Amr; Sayed-Ahmed, Ibrahim; Roongpoovapatr, Vatookarn; Abdel-Mottaleb, Mohamed; Abou Shousha, Mohamed.
In: Clinical Ophthalmology, Vol. 13, 01.01.2019, p. 781-787.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Evaluation of endothelial/Descemet membrane complex of eye bank donor corneas using enhanced depth imaging optical coherence tomography
AU - Syed, Zeba A.
AU - Gameiro, Gustavo Rosa
AU - Ruggeri, Marco
AU - Elsawy, Amr
AU - Sayed-Ahmed, Ibrahim
AU - Roongpoovapatr, Vatookarn
AU - Abdel-Mottaleb, Mohamed
AU - Abou Shousha, Mohamed
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Objective: We present a novel method for screening eye bank donor corneas using high definition optical coherence tomography (HD-OCT). This technology allows for the quantification of endothelial/Descemet membrane (En/DM) complex thickness ex vivo. Design: Prospective interventional study. Participants: Fifty-two corneal grafts from 27 donors were included in this study. Twenty additional control eyes and 11 eyes with Fuchs’ endothelial corneal dystrophy were also evaluated for comparison. Methods: A custom built, high speed HD-OCT device (Envisu R2210, Bioptigen, Buffalo Grove, IL, USA) was used to obtain images, and custom-made graph-based segmentation software was used to automatically deconstruct corneal images into micro-layers. HD-OCT imaging was used to scan through the sealed sterile case of donor corneas stored in McCarey-Kaufman medium to image their En/DM complex through the center of the cornea. Results: This technology allowed for quantification of En/DM complex thickness in all donor corneas through the sealed sterile container used to transport graft tissue. Mean En/ DM complex thickness of donor corneas was 17±4 μm. The difference between donor cornea En/DM thickness and that of control subjects (16±2 μm) was not statistically significant (p=0.3), suggesting that the transport container and media do not affect measurements. There was a significant difference between En/DM thickness of Fuchs’ endothelial corneal dystrophy eyes (25±5 μm) and both donor corneas (p<0.0001) and control subjects (p<0.0001). Conclusions: We have described a new technique to measure En/DM complex thickness in eye bank donor corneas stored in a sealed sterile case. This may represent a novel adjunctive approach to screen corneal grafts for early endothelial disease.
AB - Objective: We present a novel method for screening eye bank donor corneas using high definition optical coherence tomography (HD-OCT). This technology allows for the quantification of endothelial/Descemet membrane (En/DM) complex thickness ex vivo. Design: Prospective interventional study. Participants: Fifty-two corneal grafts from 27 donors were included in this study. Twenty additional control eyes and 11 eyes with Fuchs’ endothelial corneal dystrophy were also evaluated for comparison. Methods: A custom built, high speed HD-OCT device (Envisu R2210, Bioptigen, Buffalo Grove, IL, USA) was used to obtain images, and custom-made graph-based segmentation software was used to automatically deconstruct corneal images into micro-layers. HD-OCT imaging was used to scan through the sealed sterile case of donor corneas stored in McCarey-Kaufman medium to image their En/DM complex through the center of the cornea. Results: This technology allowed for quantification of En/DM complex thickness in all donor corneas through the sealed sterile container used to transport graft tissue. Mean En/ DM complex thickness of donor corneas was 17±4 μm. The difference between donor cornea En/DM thickness and that of control subjects (16±2 μm) was not statistically significant (p=0.3), suggesting that the transport container and media do not affect measurements. There was a significant difference between En/DM thickness of Fuchs’ endothelial corneal dystrophy eyes (25±5 μm) and both donor corneas (p<0.0001) and control subjects (p<0.0001). Conclusions: We have described a new technique to measure En/DM complex thickness in eye bank donor corneas stored in a sealed sterile case. This may represent a novel adjunctive approach to screen corneal grafts for early endothelial disease.
KW - Corneal graft screening
KW - Corneal transplant
KW - Fuchs’ endothelial corneal dystrophy
KW - Microscope-integrated OCT
UR - http://www.scopus.com/inward/record.url?scp=85067584764&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85067584764&partnerID=8YFLogxK
U2 - 10.2147/OPTH.S185455
DO - 10.2147/OPTH.S185455
M3 - Article
AN - SCOPUS:85067584764
VL - 13
SP - 781
EP - 787
JO - Clinical Ophthalmology
JF - Clinical Ophthalmology
SN - 1177-5467
ER -