Evaluation of attractive toxic sugar bait (ATSB)-Barrier for control of vector and nuisance mosquitoes and its effect on non-target organisms in sub-tropical environments in Florida

Whitney A. Qualls, Günter C. Müller, Edita E. Revay, Sandra A. Allan, Kristopher Arheart, John C Beier, Michal L. Smith, Jodi M. Scott, Vasiliy D. Kravchenko, Axel Hausmann, Zoya A. Yefremova, Rui De Xue

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The efficacy of attractive toxic sugar baits (ATSB) with the active ingredient eugenol, an Environmental Protection Agency exempt compound, was evaluated against vector and nuisance mosquitoes in both laboratory and field studies. In the laboratory, eugenol combined in attractive sugar bait (ASB) solution provided high levels of mortality for Aedes aegypti, Culex quinquefasciatus, and Anopheles quadrimaculatus. Field studies demonstrated significant control: >70% reduction for Aedes atlanticus, Aedes. infirmatus, and Culex nigripalpus and >50% reduction for Anopheles crucians, Uranotaenia sapphirina, Culiseta melanura, and Culex erraticus three weeks post ATSB application. Furthermore, non-target feeding of six insect orders, Hymenoptera, Lepidoptera, Coleoptera, Diptera, Hemiptera, and Orthoptera, was evaluated in the field after application of a dyed-ASB to flowering and non-flowering vegetation. ASB feeding (staining) was determined by dissecting the guts and searching for food dye with a dissecting microscope. The potential impact of ATSB on non-targets, applied on green non-flowering vegetation was low for all non-target groups (0.9%). However, application of the ASB to flowering vegetation resulted in significant staining of the non-target insect orders. This highlights the need for application guidelines to reduce non-target effects. No mortality was observed in laboratory studies with predatory non-targets, spiders, praying mantis, or ground beetles, after feeding for three days on mosquitoes engorged on ATSB. Overall, our laboratory and field studies support the use of eugenol as an active ingredient for controlling important vector and nuisance mosquitoes when used as an ATSB toxin. This is the first study demonstrating effective control of anophelines in non-arid environments which suggest that even in highly competitive sugar rich environments this method could be used for control of malaria in Latin American countries.

Original languageEnglish
Pages (from-to)104-110
Number of pages7
JournalActa Tropica
Volume131
Issue number1
DOIs
StatePublished - Mar 1 2014

Fingerprint

vector control
Poisons
nontarget organisms
baits
Culicidae
Eugenol
Culex
Aedes
sugars
Anopheles
Beetles
Insects
eugenol
Mantodea
Orthoptera
Staining and Labeling
United States Environmental Protection Agency
Hemiptera
Hymenoptera
Lepidoptera

Keywords

  • Anopheles crucians
  • Eugenol
  • Integrated vector control
  • Oral insecticide
  • Sugar feeding

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Evaluation of attractive toxic sugar bait (ATSB)-Barrier for control of vector and nuisance mosquitoes and its effect on non-target organisms in sub-tropical environments in Florida. / Qualls, Whitney A.; Müller, Günter C.; Revay, Edita E.; Allan, Sandra A.; Arheart, Kristopher; Beier, John C; Smith, Michal L.; Scott, Jodi M.; Kravchenko, Vasiliy D.; Hausmann, Axel; Yefremova, Zoya A.; Xue, Rui De.

In: Acta Tropica, Vol. 131, No. 1, 01.03.2014, p. 104-110.

Research output: Contribution to journalArticle

Qualls, WA, Müller, GC, Revay, EE, Allan, SA, Arheart, K, Beier, JC, Smith, ML, Scott, JM, Kravchenko, VD, Hausmann, A, Yefremova, ZA & Xue, RD 2014, 'Evaluation of attractive toxic sugar bait (ATSB)-Barrier for control of vector and nuisance mosquitoes and its effect on non-target organisms in sub-tropical environments in Florida', Acta Tropica, vol. 131, no. 1, pp. 104-110. https://doi.org/10.1016/j.actatropica.2013.12.004
Qualls, Whitney A. ; Müller, Günter C. ; Revay, Edita E. ; Allan, Sandra A. ; Arheart, Kristopher ; Beier, John C ; Smith, Michal L. ; Scott, Jodi M. ; Kravchenko, Vasiliy D. ; Hausmann, Axel ; Yefremova, Zoya A. ; Xue, Rui De. / Evaluation of attractive toxic sugar bait (ATSB)-Barrier for control of vector and nuisance mosquitoes and its effect on non-target organisms in sub-tropical environments in Florida. In: Acta Tropica. 2014 ; Vol. 131, No. 1. pp. 104-110.
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