Evaluation of a novel serotyping system for hepatitis C virus: Strong correlation with standard genotyping methodologies

V. Dixit, S. Quan, Paul Martin, D. Larson, M. Brezina, R. DiNello, K. Sra, J. Y N Lau, D. Chien, J. Kolberg, A. Tagger, G. Davis, A. Polito, G. Gitnick

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Direct sequencing and analysis of viral genomes are definitive methods for identifying various hepatitis C virus (HCV) genotypes. However, HCV genome sequencing methods are cumbersome and unsuitable for analyzing large numbers of clinical samples. We have developed a convenient, reliable, and reproducible RIBA strip immunoblot assay system for determining HCV serotype. Briefly, the assay consists of an immunoblot strip on which there are five lanes of immobilized serotype-specific HCV peptides from the nonstructural (NS-4) and core regions of the genomes of HCV types 1, 2, and 3. HCV serotype is deduced by determining the greatest intensity of reactivity to the NS-4 serotype-specific HCV peptide band in relation to the intensity of the human immunoglobulin G internal control bands on each strip. HCV core peptide reactivity is used only in the absence of NS-4 reactivity. We used this assay to successfully serotype a high percentage of sera from well-documented HCV- infected patients. Our serotyping results correlated 99% with the findings from the standard restriction fragment length polymorphism genotyping methods. Less than 5% of the serum samples were untypeable. For a selected group of alpha interferon-treated patients we observed that the nonresponders (76.2%) and a majority of the responders who relapsed (72.2%) had type 1 HCV infection. A small population (n = 8) of complete responders was split 3:4:1 as type 1, type 2, and type 3, respectively. Our data indicate that this new serotyping assay has the potential to be a highly specific and reliable method for typing of HCV infection in patients.

Original languageEnglish
Pages (from-to)2978-2983
Number of pages6
JournalJournal of Clinical Microbiology
Volume33
Issue number11
StatePublished - Jan 1 1995
Externally publishedYes

Fingerprint

Serotyping
Hepacivirus
Virus Diseases
Peptides
Genome
Viral Genome
Serum
Interferon-alpha
Restriction Fragment Length Polymorphisms
Immunoglobulin G
Genotype
Serogroup

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Evaluation of a novel serotyping system for hepatitis C virus : Strong correlation with standard genotyping methodologies. / Dixit, V.; Quan, S.; Martin, Paul; Larson, D.; Brezina, M.; DiNello, R.; Sra, K.; Lau, J. Y N; Chien, D.; Kolberg, J.; Tagger, A.; Davis, G.; Polito, A.; Gitnick, G.

In: Journal of Clinical Microbiology, Vol. 33, No. 11, 01.01.1995, p. 2978-2983.

Research output: Contribution to journalArticle

Dixit, V, Quan, S, Martin, P, Larson, D, Brezina, M, DiNello, R, Sra, K, Lau, JYN, Chien, D, Kolberg, J, Tagger, A, Davis, G, Polito, A & Gitnick, G 1995, 'Evaluation of a novel serotyping system for hepatitis C virus: Strong correlation with standard genotyping methodologies', Journal of Clinical Microbiology, vol. 33, no. 11, pp. 2978-2983.
Dixit, V. ; Quan, S. ; Martin, Paul ; Larson, D. ; Brezina, M. ; DiNello, R. ; Sra, K. ; Lau, J. Y N ; Chien, D. ; Kolberg, J. ; Tagger, A. ; Davis, G. ; Polito, A. ; Gitnick, G. / Evaluation of a novel serotyping system for hepatitis C virus : Strong correlation with standard genotyping methodologies. In: Journal of Clinical Microbiology. 1995 ; Vol. 33, No. 11. pp. 2978-2983.
@article{f80ca04bb4754187b9b455f14322191d,
title = "Evaluation of a novel serotyping system for hepatitis C virus: Strong correlation with standard genotyping methodologies",
abstract = "Direct sequencing and analysis of viral genomes are definitive methods for identifying various hepatitis C virus (HCV) genotypes. However, HCV genome sequencing methods are cumbersome and unsuitable for analyzing large numbers of clinical samples. We have developed a convenient, reliable, and reproducible RIBA strip immunoblot assay system for determining HCV serotype. Briefly, the assay consists of an immunoblot strip on which there are five lanes of immobilized serotype-specific HCV peptides from the nonstructural (NS-4) and core regions of the genomes of HCV types 1, 2, and 3. HCV serotype is deduced by determining the greatest intensity of reactivity to the NS-4 serotype-specific HCV peptide band in relation to the intensity of the human immunoglobulin G internal control bands on each strip. HCV core peptide reactivity is used only in the absence of NS-4 reactivity. We used this assay to successfully serotype a high percentage of sera from well-documented HCV- infected patients. Our serotyping results correlated 99{\%} with the findings from the standard restriction fragment length polymorphism genotyping methods. Less than 5{\%} of the serum samples were untypeable. For a selected group of alpha interferon-treated patients we observed that the nonresponders (76.2{\%}) and a majority of the responders who relapsed (72.2{\%}) had type 1 HCV infection. A small population (n = 8) of complete responders was split 3:4:1 as type 1, type 2, and type 3, respectively. Our data indicate that this new serotyping assay has the potential to be a highly specific and reliable method for typing of HCV infection in patients.",
author = "V. Dixit and S. Quan and Paul Martin and D. Larson and M. Brezina and R. DiNello and K. Sra and Lau, {J. Y N} and D. Chien and J. Kolberg and A. Tagger and G. Davis and A. Polito and G. Gitnick",
year = "1995",
month = "1",
day = "1",
language = "English",
volume = "33",
pages = "2978--2983",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "11",

}

TY - JOUR

T1 - Evaluation of a novel serotyping system for hepatitis C virus

T2 - Strong correlation with standard genotyping methodologies

AU - Dixit, V.

AU - Quan, S.

AU - Martin, Paul

AU - Larson, D.

AU - Brezina, M.

AU - DiNello, R.

AU - Sra, K.

AU - Lau, J. Y N

AU - Chien, D.

AU - Kolberg, J.

AU - Tagger, A.

AU - Davis, G.

AU - Polito, A.

AU - Gitnick, G.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Direct sequencing and analysis of viral genomes are definitive methods for identifying various hepatitis C virus (HCV) genotypes. However, HCV genome sequencing methods are cumbersome and unsuitable for analyzing large numbers of clinical samples. We have developed a convenient, reliable, and reproducible RIBA strip immunoblot assay system for determining HCV serotype. Briefly, the assay consists of an immunoblot strip on which there are five lanes of immobilized serotype-specific HCV peptides from the nonstructural (NS-4) and core regions of the genomes of HCV types 1, 2, and 3. HCV serotype is deduced by determining the greatest intensity of reactivity to the NS-4 serotype-specific HCV peptide band in relation to the intensity of the human immunoglobulin G internal control bands on each strip. HCV core peptide reactivity is used only in the absence of NS-4 reactivity. We used this assay to successfully serotype a high percentage of sera from well-documented HCV- infected patients. Our serotyping results correlated 99% with the findings from the standard restriction fragment length polymorphism genotyping methods. Less than 5% of the serum samples were untypeable. For a selected group of alpha interferon-treated patients we observed that the nonresponders (76.2%) and a majority of the responders who relapsed (72.2%) had type 1 HCV infection. A small population (n = 8) of complete responders was split 3:4:1 as type 1, type 2, and type 3, respectively. Our data indicate that this new serotyping assay has the potential to be a highly specific and reliable method for typing of HCV infection in patients.

AB - Direct sequencing and analysis of viral genomes are definitive methods for identifying various hepatitis C virus (HCV) genotypes. However, HCV genome sequencing methods are cumbersome and unsuitable for analyzing large numbers of clinical samples. We have developed a convenient, reliable, and reproducible RIBA strip immunoblot assay system for determining HCV serotype. Briefly, the assay consists of an immunoblot strip on which there are five lanes of immobilized serotype-specific HCV peptides from the nonstructural (NS-4) and core regions of the genomes of HCV types 1, 2, and 3. HCV serotype is deduced by determining the greatest intensity of reactivity to the NS-4 serotype-specific HCV peptide band in relation to the intensity of the human immunoglobulin G internal control bands on each strip. HCV core peptide reactivity is used only in the absence of NS-4 reactivity. We used this assay to successfully serotype a high percentage of sera from well-documented HCV- infected patients. Our serotyping results correlated 99% with the findings from the standard restriction fragment length polymorphism genotyping methods. Less than 5% of the serum samples were untypeable. For a selected group of alpha interferon-treated patients we observed that the nonresponders (76.2%) and a majority of the responders who relapsed (72.2%) had type 1 HCV infection. A small population (n = 8) of complete responders was split 3:4:1 as type 1, type 2, and type 3, respectively. Our data indicate that this new serotyping assay has the potential to be a highly specific and reliable method for typing of HCV infection in patients.

UR - http://www.scopus.com/inward/record.url?scp=0028818807&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028818807&partnerID=8YFLogxK

M3 - Article

C2 - 8576357

AN - SCOPUS:0028818807

VL - 33

SP - 2978

EP - 2983

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 11

ER -