Evaluation of a Flow Cytometry-Based Assay for Natural Killer Cell Activity in Clinical Settings

R. Valiathan, John E Lewis, A. B. Melillo, S. Leonard, K. H. Ali, D. Asthana

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Natural killer (NK) cells are not only important in first line defence against viral and bacterial infections, but also in immune surveillance of malignant cells and thus NK cell cytotoxicity is primary indicator of immune function. Although chromium release assay is recognized as 'gold standard' for measuring NK cell activity, it has disadvantages like use of radioactive compounds, poor loading and high spontaneous release. It is difficult to perform this assay in clinical laboratory because of difficulties with disposal of radioactive waste and standardization problems. We describe a flow cytometry-based assay for the measurement of NK cell activity by incorporating fluorescent dye, DiO, into membranes of target cells. NK cell activity was measured at baseline, 1 and 4weeks follow-up in 20 normal healthy individuals on a dietary supplement immunomodulator to enhance NK cell function. Mean baseline NK cell activity percentage (21.5; SD=9.3) increased significantly to a maximum level at 1week (31.3%; SD=7.9; P=0.007) and then returned to baseline level at 4weeks (21.5; SD=8.3). An important feature of flow cytometry-based assays is its ability to discriminate effector cells from target cells, and potential for explaining molecular interactions underlying target cell lysis. Under clinical settings, this assay will be of interest for frequently monitoring immunological status of patients on treatment for various diseases that affect their immune status. The assay is easy to perform without using radioactive material and thus could become a tool for monitoring pathogenesis and immune reconstitution.

Original languageEnglish
Pages (from-to)455-462
Number of pages8
JournalScandinavian Journal of Immunology
Volume75
Issue number4
DOIs
StatePublished - Apr 1 2012

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Natural Killer Cells
Flow Cytometry
Immunologic Monitoring
Radioactive Waste
Immunologic Factors
Chromium
Virus Diseases
Dietary Supplements
Fluorescent Dyes
Bacterial Infections
Cell Membrane

ASJC Scopus subject areas

  • Immunology

Cite this

Evaluation of a Flow Cytometry-Based Assay for Natural Killer Cell Activity in Clinical Settings. / Valiathan, R.; Lewis, John E; Melillo, A. B.; Leonard, S.; Ali, K. H.; Asthana, D.

In: Scandinavian Journal of Immunology, Vol. 75, No. 4, 01.04.2012, p. 455-462.

Research output: Contribution to journalArticle

Valiathan, R. ; Lewis, John E ; Melillo, A. B. ; Leonard, S. ; Ali, K. H. ; Asthana, D. / Evaluation of a Flow Cytometry-Based Assay for Natural Killer Cell Activity in Clinical Settings. In: Scandinavian Journal of Immunology. 2012 ; Vol. 75, No. 4. pp. 455-462.
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