Evaluating Atlantic bottlenose dolphin (Tursiops truncatus) leukocyte differential counts comparing the CellaVision DM96 and the manual method

Julia Zaias, Christine Bradley, Jill Richardson, Holli C. Eskelinen, Offiong Ikpatt, Carolyn Cray

Research output: Contribution to journalArticle

Abstract

Background: The leukocyte differential count is an excellent diagnostic tool; however, the manual differential count has several drawbacks, especially for nontraditional species. Automated cell analyzers commonly used in veterinary practices require species-specific validation for use in nondomestic species other than dogs and cats. Objectives: The purpose of this study was to examine the potential of the CellaVision DM96 (DM96), an automated image analysis system, as a rapid and accurate method for providing a WBC differential count in comparison to the manual WBC differential count in bottlenose dolphins. Methods: Ten fresh, EDTA anticoagulated blood samples were collected, blood smears were made and stained, and the differential WBC counts were performed on the DM96 and compared with manual differential WBC counts. Agreement, means, and errors were compared between the methods. Results: There was good agreement between the DM96 and manual differential WBC counts for neutrophils; however, there was significant variation when comparing lymphocyte, monocyte, and eosinophil counts. No basophils were seen by any method. Conclusions: Despite a small sample size, the DM96 appeared to provide a viable alternative for automated neutrophil counting in blood of bottlenose dolphins. Whether the counts are comparable in animals with highly pathologic differential counts must be addressed in follow-up studies, preferably with more study animals.

Original languageEnglish (US)
JournalVeterinary Clinical Pathology
DOIs
StateAccepted/In press - 2017

Fingerprint

Bottle-Nosed Dolphin
Tursiops truncatus
Leukocyte Count
leukocyte count
neutrophils
leukocytes
blood
Neutrophils
basophils
Basophils
eosinophils
methodology
Eosinophils
Edetic Acid
monocytes
Sample Size
Monocytes
animals
Cats
lymphocytes

Keywords

  • Automated leukocyte differential
  • CellaVision DM96
  • Dolphin

ASJC Scopus subject areas

  • veterinary(all)

Cite this

@article{e3dbe624a7b44ec59e8ae5cf2368287c,
title = "Evaluating Atlantic bottlenose dolphin (Tursiops truncatus) leukocyte differential counts comparing the CellaVision DM96 and the manual method",
abstract = "Background: The leukocyte differential count is an excellent diagnostic tool; however, the manual differential count has several drawbacks, especially for nontraditional species. Automated cell analyzers commonly used in veterinary practices require species-specific validation for use in nondomestic species other than dogs and cats. Objectives: The purpose of this study was to examine the potential of the CellaVision DM96 (DM96), an automated image analysis system, as a rapid and accurate method for providing a WBC differential count in comparison to the manual WBC differential count in bottlenose dolphins. Methods: Ten fresh, EDTA anticoagulated blood samples were collected, blood smears were made and stained, and the differential WBC counts were performed on the DM96 and compared with manual differential WBC counts. Agreement, means, and errors were compared between the methods. Results: There was good agreement between the DM96 and manual differential WBC counts for neutrophils; however, there was significant variation when comparing lymphocyte, monocyte, and eosinophil counts. No basophils were seen by any method. Conclusions: Despite a small sample size, the DM96 appeared to provide a viable alternative for automated neutrophil counting in blood of bottlenose dolphins. Whether the counts are comparable in animals with highly pathologic differential counts must be addressed in follow-up studies, preferably with more study animals.",
keywords = "Automated leukocyte differential, CellaVision DM96, Dolphin",
author = "Julia Zaias and Christine Bradley and Jill Richardson and Eskelinen, {Holli C.} and Offiong Ikpatt and Carolyn Cray",
year = "2017",
doi = "10.1111/vcp.12514",
language = "English (US)",
journal = "Veterinary Clinical Pathology",
issn = "0275-6382",
publisher = "American Society for Veterinary Clinical Pathology",

}

TY - JOUR

T1 - Evaluating Atlantic bottlenose dolphin (Tursiops truncatus) leukocyte differential counts comparing the CellaVision DM96 and the manual method

AU - Zaias, Julia

AU - Bradley, Christine

AU - Richardson, Jill

AU - Eskelinen, Holli C.

AU - Ikpatt, Offiong

AU - Cray, Carolyn

PY - 2017

Y1 - 2017

N2 - Background: The leukocyte differential count is an excellent diagnostic tool; however, the manual differential count has several drawbacks, especially for nontraditional species. Automated cell analyzers commonly used in veterinary practices require species-specific validation for use in nondomestic species other than dogs and cats. Objectives: The purpose of this study was to examine the potential of the CellaVision DM96 (DM96), an automated image analysis system, as a rapid and accurate method for providing a WBC differential count in comparison to the manual WBC differential count in bottlenose dolphins. Methods: Ten fresh, EDTA anticoagulated blood samples were collected, blood smears were made and stained, and the differential WBC counts were performed on the DM96 and compared with manual differential WBC counts. Agreement, means, and errors were compared between the methods. Results: There was good agreement between the DM96 and manual differential WBC counts for neutrophils; however, there was significant variation when comparing lymphocyte, monocyte, and eosinophil counts. No basophils were seen by any method. Conclusions: Despite a small sample size, the DM96 appeared to provide a viable alternative for automated neutrophil counting in blood of bottlenose dolphins. Whether the counts are comparable in animals with highly pathologic differential counts must be addressed in follow-up studies, preferably with more study animals.

AB - Background: The leukocyte differential count is an excellent diagnostic tool; however, the manual differential count has several drawbacks, especially for nontraditional species. Automated cell analyzers commonly used in veterinary practices require species-specific validation for use in nondomestic species other than dogs and cats. Objectives: The purpose of this study was to examine the potential of the CellaVision DM96 (DM96), an automated image analysis system, as a rapid and accurate method for providing a WBC differential count in comparison to the manual WBC differential count in bottlenose dolphins. Methods: Ten fresh, EDTA anticoagulated blood samples were collected, blood smears were made and stained, and the differential WBC counts were performed on the DM96 and compared with manual differential WBC counts. Agreement, means, and errors were compared between the methods. Results: There was good agreement between the DM96 and manual differential WBC counts for neutrophils; however, there was significant variation when comparing lymphocyte, monocyte, and eosinophil counts. No basophils were seen by any method. Conclusions: Despite a small sample size, the DM96 appeared to provide a viable alternative for automated neutrophil counting in blood of bottlenose dolphins. Whether the counts are comparable in animals with highly pathologic differential counts must be addressed in follow-up studies, preferably with more study animals.

KW - Automated leukocyte differential

KW - CellaVision DM96

KW - Dolphin

UR - http://www.scopus.com/inward/record.url?scp=85020465298&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020465298&partnerID=8YFLogxK

U2 - 10.1111/vcp.12514

DO - 10.1111/vcp.12514

M3 - Article

JO - Veterinary Clinical Pathology

JF - Veterinary Clinical Pathology

SN - 0275-6382

ER -