EUS-guided fine-needle aspiration combined with flow cytometry and immunocytochemistry in the diagnosis of lymphoma

Afonso Ribeiro, Enrique Vazquez-Sequeiros, Lisa M. Wiersema, Kenneth K. Wang, Jonathan E. Clain, Maurits J. Wiersema

Research output: Contribution to journalArticle

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Abstract

Limited information is available regarding the use of EUS-guided fine-needle aspiration (EUS-FNA) in the diagnosis of lymphoproliferative disorders. The aim of this study was to evaluate the yield of this technique in the primary diagnosis of lymphoma. The records were reviewed of 38 consecutive patients with GI lesions and/or enlarged lymph nodes identified on imaging studies that raised a suspicion of lymphoma who underwent EUS-FNA of lymph nodes or the gut wall. Final diagnosis was based on clinical follow-up, imaging studies, or surgical findings. Twenty-three patients with lymphoma and 15 patients with benign disease or reactive lymphadenopathy were identified. The overall sensitivity, specificity, and accuracy of EUS-FNA cytology with flow cytometry/immunocytochemistry (FC/IC) for the diagnosis of lymphoma were, respectively, 74%, 93%, and 81%. When comparing patients who had EUS-FNA with FC/IC versus those who had EUS-FNA without FC/IC, sensitivity was 86% versus 44% (p = 0.04), specificity was 100% versus 90% (not significant), and accuracy was 89% versus 68% (not significant). EUS-FNA can provide cytology specimens diagnostic for lymphoma. Selective use of FC/IC in patients with suspected lymphoma improves the yield of EUS-FNA and may guide diagnostic evaluation and treatment decisions.

Original languageEnglish
Pages (from-to)485-491
Number of pages7
JournalGastrointestinal Endoscopy
Volume53
Issue number4
DOIs
StatePublished - Apr 1 2001
Externally publishedYes

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Fine Needle Biopsy
Lymphoma
Flow Cytometry
Immunohistochemistry
Cell Biology
Lymph Nodes
Lymphoproliferative Disorders
Sensitivity and Specificity

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Ribeiro, A., Vazquez-Sequeiros, E., Wiersema, L. M., Wang, K. K., Clain, J. E., & Wiersema, M. J. (2001). EUS-guided fine-needle aspiration combined with flow cytometry and immunocytochemistry in the diagnosis of lymphoma. Gastrointestinal Endoscopy, 53(4), 485-491. https://doi.org/10.1067/mge.2001.112841

EUS-guided fine-needle aspiration combined with flow cytometry and immunocytochemistry in the diagnosis of lymphoma. / Ribeiro, Afonso; Vazquez-Sequeiros, Enrique; Wiersema, Lisa M.; Wang, Kenneth K.; Clain, Jonathan E.; Wiersema, Maurits J.

In: Gastrointestinal Endoscopy, Vol. 53, No. 4, 01.04.2001, p. 485-491.

Research output: Contribution to journalArticle

Ribeiro, A, Vazquez-Sequeiros, E, Wiersema, LM, Wang, KK, Clain, JE & Wiersema, MJ 2001, 'EUS-guided fine-needle aspiration combined with flow cytometry and immunocytochemistry in the diagnosis of lymphoma', Gastrointestinal Endoscopy, vol. 53, no. 4, pp. 485-491. https://doi.org/10.1067/mge.2001.112841
Ribeiro, Afonso ; Vazquez-Sequeiros, Enrique ; Wiersema, Lisa M. ; Wang, Kenneth K. ; Clain, Jonathan E. ; Wiersema, Maurits J. / EUS-guided fine-needle aspiration combined with flow cytometry and immunocytochemistry in the diagnosis of lymphoma. In: Gastrointestinal Endoscopy. 2001 ; Vol. 53, No. 4. pp. 485-491.
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