Ethanol inhibits human bone cell proliferation and function in vitro

Karen E. Friday, Guy Howard

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

The direct effects of ethanol on human bone cell proliferation and function were studied in vitro. Normal human osteoblasts from trabecular bone chips were prepared by collagenase digestion. Exposure of these osteoblasts to ethanol in concentrations of 0.05% to 1% for 22 hours induced a dose-dependent reduction in bone cell DNA synthesis as assessed by incorporation of 3H-thymidine. After 72 hours of ethanol exposure in concentrations of 0.01% to 1%, protein synthesis as measured by 3H-proline incorporation into trichbroacetic acid (TCA)-precipitable material was reduced in a dose-dependent manner. Human bone cell protein concentrations and alkaline phosphatase total activity were significantly reduced after exposure to 1% ethanol for 72 hours, but not with lower concentrations of ethanol. This reduction in osteoblast proliferation and activity may partially explain the development of osteopenia in humans consuming excessive amounts of ethanol.

Original languageEnglish
Pages (from-to)562-565
Number of pages4
JournalMetabolism
Volume40
Issue number6
DOIs
StatePublished - Jan 1 1991
Externally publishedYes

Fingerprint

Ethanol
Cell Proliferation
Bone and Bones
Osteoblasts
Metabolic Bone Diseases
Phosphoprotein Phosphatases
Collagenases
Proline
Thymidine
Alkaline Phosphatase
In Vitro Techniques
Digestion
Acids
DNA
Proteins

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Ethanol inhibits human bone cell proliferation and function in vitro. / Friday, Karen E.; Howard, Guy.

In: Metabolism, Vol. 40, No. 6, 01.01.1991, p. 562-565.

Research output: Contribution to journalArticle

Friday, Karen E. ; Howard, Guy. / Ethanol inhibits human bone cell proliferation and function in vitro. In: Metabolism. 1991 ; Vol. 40, No. 6. pp. 562-565.
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