Ethanol effects on synaptic glutamate receptor function and on membrane lipid organization

E. K. Michaelis, H. H. Chang, S. Roy, J. A. McFaul, J. D. Zimbrick

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

The enhancement of L-glutamic acid binding activity of brain synaptic membranes by low concentrations of ethanol (<50 mM) and the decrease in binding at high concentrations (>100 mM) was not due to a direct action by ethanol on the glutamate binding protein. Biphasis effects of ethanol on membrane protein complexes such as the glutamate binding sites might be the result of biphasic changes in membrane lipid organization. Low ethanol concentrations (0.1-4.0 mM) were shown to decrease fatty acid chain motion detected by the EPR probe 5-doxyl stearic acid, whereas high concentrations (>400 mM) increased lipid motion in egg phosphatidylcholine liposomes. The function of the L-glutamate receptor-ion channel complex in the presence of ethanol was also determined by measuring the changes in thiocyanate (SCN-) influx brought about by L-glutamate or ethanol. A low concentration of ethanol (9.4 mM) diminished the L-glutamate-induced depolarization of synaptic membranes, while a high concetration (93.7 mM) increased the passive SCN- influx and produced a transient overshoot in glutamate-stimulated SCN- flux.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalPharmacology, Biochemistry and Behavior
Volume18
Issue numberSUPPL. 1
DOIs
StatePublished - 1983
Externally publishedYes

Keywords

  • EPR
  • Ethanol
  • Glutamate receptors
  • Liposomes
  • Membrane lipids
  • Membrane structure
  • Synaptic membranes

ASJC Scopus subject areas

  • Biochemistry
  • Toxicology
  • Pharmacology
  • Clinical Biochemistry
  • Biological Psychiatry
  • Behavioral Neuroscience

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