Estrogen regulation of Xenopus laevis γ-fibrinogen gene expression

Ricardo Pastori, John E. Moskaitis, Laurens H. Smith, Daniel R. Schoenberg

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Albumin gene expression in Xenopus is regulated by estrogen through changes in the stability of its mRNA. The goal of the present study was to determine whether a similar pathway regulates γ-fibrinogen. Degenerate oligonucleotides directed to conserved regions of the carboxyl-terminal half (domain D) of human and lamprey γ-fibrinogen were used to isolate a full-length cDNA clone for Xenopus γ-fibrinogen. Analysis of codon utilization from the DNA sequence of this clone revealed that Xenopus γ-fibrinogen mRNA shows the same bias against CG dinucleotides as present in human, but not lamprey, fibrinogen mRNA. Features of the protein shared with the human homologue include all of the cysteine residues, an N-linked glycosylation site at amino acid 50, and 75% sequence identity in domain D. Much of the same region is conserved in lamprey γ-fibrinogen. There is only a single size mRNA encoding γ-fibrinogen in Xenopus, unlike rats where two mRNAs of different length are generated by alternate splicing. Administration of estrogen to male Xenopus results in the disappearance of γ-fibrinogen mRNA from the cytoplasm, with no effect on steady-state levels in the nucleus. This process can be blocked by prior treatment with anti-estrogen, indicating that, like the regulation of serum albumin mRNA, γ-fibrinogen is regulated posttranscriptionally through an estrogen receptor dependent mechanism. It is postulated that a consensus sequence flanking the AAUAAA polyadenylation signal in γ-fibrinogen and the 68- and 74-kDa albumin mRNAs, but not vitellogenin or β-globin mRNA, may play a role in the hormonal regulation of mRNA stability.

Original languageEnglish
Pages (from-to)2599-2605
Number of pages7
JournalBiochemistry
Volume29
Issue number10
StatePublished - Dec 1 1990
Externally publishedYes

Fingerprint

Xenopus laevis
Gene expression
Fibrinogen
Estrogens
Gene Expression
Messenger RNA
Xenopus
Lampreys
RNA Stability
Albumins
Clone Cells
Glycosylation
Vitellogenins
Polyadenylation
Globins
DNA sequences
Consensus Sequence
Alternative Splicing
Serum Albumin
Codon

ASJC Scopus subject areas

  • Biochemistry

Cite this

Pastori, R., Moskaitis, J. E., Smith, L. H., & Schoenberg, D. R. (1990). Estrogen regulation of Xenopus laevis γ-fibrinogen gene expression. Biochemistry, 29(10), 2599-2605.

Estrogen regulation of Xenopus laevis γ-fibrinogen gene expression. / Pastori, Ricardo; Moskaitis, John E.; Smith, Laurens H.; Schoenberg, Daniel R.

In: Biochemistry, Vol. 29, No. 10, 01.12.1990, p. 2599-2605.

Research output: Contribution to journalArticle

Pastori, R, Moskaitis, JE, Smith, LH & Schoenberg, DR 1990, 'Estrogen regulation of Xenopus laevis γ-fibrinogen gene expression', Biochemistry, vol. 29, no. 10, pp. 2599-2605.
Pastori R, Moskaitis JE, Smith LH, Schoenberg DR. Estrogen regulation of Xenopus laevis γ-fibrinogen gene expression. Biochemistry. 1990 Dec 1;29(10):2599-2605.
Pastori, Ricardo ; Moskaitis, John E. ; Smith, Laurens H. ; Schoenberg, Daniel R. / Estrogen regulation of Xenopus laevis γ-fibrinogen gene expression. In: Biochemistry. 1990 ; Vol. 29, No. 10. pp. 2599-2605.
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