Abstract
A quick and easy method of sucrose density gradient analysis is described in which prior preparation of gradients for protein separation is not required. We compare the refractive indices as well as the separation pattern of radioactively labeled proteins with either preformed gradients (prepared by layering or by gradient maker) or gradients that establish themselves during centrifugation. Our results show that an identical pattern of protein separation can be achieved by layering the nuclear extracted estrogen receptor either on top of one single sucrose concentration or on a preformed gradient. Centrifugation times are 40 hr in a swinging bucket type rotor (Sw60) or 15 hr in a vertical tube rotor (VTi 65). This method avoids the onerous task of gradient preparation, and the separation itself can be achieved in a 15 hr centrifugation by use of a vertical tube rotor.
| Original language | English |
|---|---|
| Pages (from-to) | 47-50 |
| Number of pages | 4 |
| Journal | Breast Cancer Research and Treatment |
| Volume | 5 |
| Issue number | 1 |
| DOIs | |
| State | Published - Feb 1 1985 |
| Externally published | Yes |
Fingerprint
Keywords
- deuterium oxide
- estrogen receptor
- protein separation
- sucrose density gradient sedimentation
ASJC Scopus subject areas
- Oncology
- Cancer Research
Cite this
Estrogen receptor separation employing sucrose deuterium oxide gradients generated during sample centrifugation. / Scholl, Susy; Lippman, Marc E.
In: Breast Cancer Research and Treatment, Vol. 5, No. 1, 01.02.1985, p. 47-50.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Estrogen receptor separation employing sucrose deuterium oxide gradients generated during sample centrifugation
AU - Scholl, Susy
AU - Lippman, Marc E
PY - 1985/2/1
Y1 - 1985/2/1
N2 - A quick and easy method of sucrose density gradient analysis is described in which prior preparation of gradients for protein separation is not required. We compare the refractive indices as well as the separation pattern of radioactively labeled proteins with either preformed gradients (prepared by layering or by gradient maker) or gradients that establish themselves during centrifugation. Our results show that an identical pattern of protein separation can be achieved by layering the nuclear extracted estrogen receptor either on top of one single sucrose concentration or on a preformed gradient. Centrifugation times are 40 hr in a swinging bucket type rotor (Sw60) or 15 hr in a vertical tube rotor (VTi 65). This method avoids the onerous task of gradient preparation, and the separation itself can be achieved in a 15 hr centrifugation by use of a vertical tube rotor.
AB - A quick and easy method of sucrose density gradient analysis is described in which prior preparation of gradients for protein separation is not required. We compare the refractive indices as well as the separation pattern of radioactively labeled proteins with either preformed gradients (prepared by layering or by gradient maker) or gradients that establish themselves during centrifugation. Our results show that an identical pattern of protein separation can be achieved by layering the nuclear extracted estrogen receptor either on top of one single sucrose concentration or on a preformed gradient. Centrifugation times are 40 hr in a swinging bucket type rotor (Sw60) or 15 hr in a vertical tube rotor (VTi 65). This method avoids the onerous task of gradient preparation, and the separation itself can be achieved in a 15 hr centrifugation by use of a vertical tube rotor.
KW - deuterium oxide
KW - estrogen receptor
KW - protein separation
KW - sucrose density gradient sedimentation
UR - http://www.scopus.com/inward/record.url?scp=0021911634&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021911634&partnerID=8YFLogxK
U2 - 10.1007/BF01807649
DO - 10.1007/BF01807649
M3 - Article
C2 - 2983797
AN - SCOPUS:0021911634
VL - 5
SP - 47
EP - 50
JO - Breast Cancer Research and Treatment
JF - Breast Cancer Research and Treatment
SN - 0167-6806
IS - 1
ER -