Estrogen receptor separation employing sucrose deuterium oxide gradients generated during sample centrifugation

Susy Scholl, Marc E Lippman

Research output: Contribution to journalArticle

Abstract

A quick and easy method of sucrose density gradient analysis is described in which prior preparation of gradients for protein separation is not required. We compare the refractive indices as well as the separation pattern of radioactively labeled proteins with either preformed gradients (prepared by layering or by gradient maker) or gradients that establish themselves during centrifugation. Our results show that an identical pattern of protein separation can be achieved by layering the nuclear extracted estrogen receptor either on top of one single sucrose concentration or on a preformed gradient. Centrifugation times are 40 hr in a swinging bucket type rotor (Sw60) or 15 hr in a vertical tube rotor (VTi 65). This method avoids the onerous task of gradient preparation, and the separation itself can be achieved in a 15 hr centrifugation by use of a vertical tube rotor.

Original languageEnglish
Pages (from-to)47-50
Number of pages4
JournalBreast Cancer Research and Treatment
Volume5
Issue number1
DOIs
StatePublished - Feb 1 1985
Externally publishedYes

Fingerprint

Deuterium Oxide
Centrifugation
Estrogen Receptors
Sucrose
Refractometry
Proteins

Keywords

  • deuterium oxide
  • estrogen receptor
  • protein separation
  • sucrose density gradient sedimentation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Estrogen receptor separation employing sucrose deuterium oxide gradients generated during sample centrifugation. / Scholl, Susy; Lippman, Marc E.

In: Breast Cancer Research and Treatment, Vol. 5, No. 1, 01.02.1985, p. 47-50.

Research output: Contribution to journalArticle

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