Estrogen inhibits vaginal tropoelastin and TGF-β1 production

Nahida Chakhtoura, Yanping Zhang, Keith Candiotti, Carlos A. Medina, Peter Takacs

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Introduction and hypothesis: The aim of this study was to quantify the effects of estrogen on vaginal smooth muscle cell (SMC) tropoelastin and transforming growth factor (TGF)-β1 production. Methods: Primary SMC were incubated with estradiol, and cell proliferation was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay at 48 h. Supernatants were collected and tropoelastin and TGF-β1 levels measured. Results: SMC proliferation was significantly increased by estradiol [relative cell number, mean ± standard error (SE), estradiol 0.1 μM 116 ± 19 % of control (P = NS), 1 μM 127 ± 13 % of control (P < 0.05), 10 μM 153 ± 26 % of control, (P < 0.05)]. Tropoelastin production was significantly decreased by estrogen [mean ± SE, estradiol 0.1 μM 78 ± 2 % of control (P < 0.05), 1 μM 76 ± 4 % of control (P < 0.05), 10 μM 67 ± 3 % of control, (P < 0.05)]. In addition, TGF-β1 production was significantly decreased [mean ± SE, estradiol 0.1 μM 96 ± 4 % of control (P = NS), 1 μM 84 ± 6 % of control (P < 0.05), 10 μM 70 ± 6 % of control, (P < 0.05)]. Conclusion: Estrogen increases vaginal SMC proliferation and inhibits tropoelastin and TGF-β1 production.

Original languageEnglish (US)
Pages (from-to)1791-1795
Number of pages5
JournalInternational Urogynecology Journal and Pelvic Floor Dysfunction
Volume23
Issue number12
DOIs
StatePublished - Dec 1 2012

Keywords

  • Elastin
  • Estrogen
  • Transforming growth factor β1
  • Vagina

ASJC Scopus subject areas

  • Urology
  • Obstetrics and Gynecology

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