TY - JOUR
T1 - Estrogen inhibits vaginal tropoelastin and TGF-β1 production
AU - Chakhtoura, Nahida
AU - Zhang, Yanping
AU - Candiotti, Keith
AU - Medina, Carlos A.
AU - Takacs, Peter
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2012/12
Y1 - 2012/12
N2 - Introduction and hypothesis: The aim of this study was to quantify the effects of estrogen on vaginal smooth muscle cell (SMC) tropoelastin and transforming growth factor (TGF)-β1 production. Methods: Primary SMC were incubated with estradiol, and cell proliferation was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay at 48 h. Supernatants were collected and tropoelastin and TGF-β1 levels measured. Results: SMC proliferation was significantly increased by estradiol [relative cell number, mean ± standard error (SE), estradiol 0.1 μM 116 ± 19 % of control (P = NS), 1 μM 127 ± 13 % of control (P < 0.05), 10 μM 153 ± 26 % of control, (P < 0.05)]. Tropoelastin production was significantly decreased by estrogen [mean ± SE, estradiol 0.1 μM 78 ± 2 % of control (P < 0.05), 1 μM 76 ± 4 % of control (P < 0.05), 10 μM 67 ± 3 % of control, (P < 0.05)]. In addition, TGF-β1 production was significantly decreased [mean ± SE, estradiol 0.1 μM 96 ± 4 % of control (P = NS), 1 μM 84 ± 6 % of control (P < 0.05), 10 μM 70 ± 6 % of control, (P < 0.05)]. Conclusion: Estrogen increases vaginal SMC proliferation and inhibits tropoelastin and TGF-β1 production.
AB - Introduction and hypothesis: The aim of this study was to quantify the effects of estrogen on vaginal smooth muscle cell (SMC) tropoelastin and transforming growth factor (TGF)-β1 production. Methods: Primary SMC were incubated with estradiol, and cell proliferation was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay at 48 h. Supernatants were collected and tropoelastin and TGF-β1 levels measured. Results: SMC proliferation was significantly increased by estradiol [relative cell number, mean ± standard error (SE), estradiol 0.1 μM 116 ± 19 % of control (P = NS), 1 μM 127 ± 13 % of control (P < 0.05), 10 μM 153 ± 26 % of control, (P < 0.05)]. Tropoelastin production was significantly decreased by estrogen [mean ± SE, estradiol 0.1 μM 78 ± 2 % of control (P < 0.05), 1 μM 76 ± 4 % of control (P < 0.05), 10 μM 67 ± 3 % of control, (P < 0.05)]. In addition, TGF-β1 production was significantly decreased [mean ± SE, estradiol 0.1 μM 96 ± 4 % of control (P = NS), 1 μM 84 ± 6 % of control (P < 0.05), 10 μM 70 ± 6 % of control, (P < 0.05)]. Conclusion: Estrogen increases vaginal SMC proliferation and inhibits tropoelastin and TGF-β1 production.
KW - Elastin
KW - Estrogen
KW - Transforming growth factor β1
KW - Vagina
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U2 - 10.1007/s00192-012-1828-6
DO - 10.1007/s00192-012-1828-6
M3 - Article
C2 - 22669426
AN - SCOPUS:84877603554
VL - 23
SP - 1791
EP - 1795
JO - International Urogynecology Journal and Pelvic Floor Dysfunction
JF - International Urogynecology Journal and Pelvic Floor Dysfunction
SN - 0937-3462
IS - 12
ER -