Abstract
Introduction and hypothesis: The aim of this study was to quantify the effects of estrogen on vaginal smooth muscle cell (SMC) tropoelastin and transforming growth factor (TGF)-β1 production. Methods: Primary SMC were incubated with estradiol, and cell proliferation was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay at 48 h. Supernatants were collected and tropoelastin and TGF-β1 levels measured. Results: SMC proliferation was significantly increased by estradiol [relative cell number, mean ± standard error (SE), estradiol 0.1 μM 116 ± 19 % of control (P = NS), 1 μM 127 ± 13 % of control (P < 0.05), 10 μM 153 ± 26 % of control, (P < 0.05)]. Tropoelastin production was significantly decreased by estrogen [mean ± SE, estradiol 0.1 μM 78 ± 2 % of control (P < 0.05), 1 μM 76 ± 4 % of control (P < 0.05), 10 μM 67 ± 3 % of control, (P < 0.05)]. In addition, TGF-β1 production was significantly decreased [mean ± SE, estradiol 0.1 μM 96 ± 4 % of control (P = NS), 1 μM 84 ± 6 % of control (P < 0.05), 10 μM 70 ± 6 % of control, (P < 0.05)]. Conclusion: Estrogen increases vaginal SMC proliferation and inhibits tropoelastin and TGF-β1 production.
| Original language | English |
|---|---|
| Pages (from-to) | 1791-1795 |
| Number of pages | 5 |
| Journal | International Urogynecology Journal and Pelvic Floor Dysfunction |
| Volume | 23 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 1 2012 |
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Keywords
- Elastin
- Estrogen
- Transforming growth factor β1
- Vagina
ASJC Scopus subject areas
- Urology
- Obstetrics and Gynecology
Cite this
Estrogen inhibits vaginal tropoelastin and TGF-β1 production. / Chakhtoura, Nahida; Zhang, Yanping; Candiotti, Keith A; Medina, Carlos; Takacs, Peter.
In: International Urogynecology Journal and Pelvic Floor Dysfunction, Vol. 23, No. 12, 01.12.2012, p. 1791-1795.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Estrogen inhibits vaginal tropoelastin and TGF-β1 production
AU - Chakhtoura, Nahida
AU - Zhang, Yanping
AU - Candiotti, Keith A
AU - Medina, Carlos
AU - Takacs, Peter
PY - 2012/12/1
Y1 - 2012/12/1
N2 - Introduction and hypothesis: The aim of this study was to quantify the effects of estrogen on vaginal smooth muscle cell (SMC) tropoelastin and transforming growth factor (TGF)-β1 production. Methods: Primary SMC were incubated with estradiol, and cell proliferation was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay at 48 h. Supernatants were collected and tropoelastin and TGF-β1 levels measured. Results: SMC proliferation was significantly increased by estradiol [relative cell number, mean ± standard error (SE), estradiol 0.1 μM 116 ± 19 % of control (P = NS), 1 μM 127 ± 13 % of control (P < 0.05), 10 μM 153 ± 26 % of control, (P < 0.05)]. Tropoelastin production was significantly decreased by estrogen [mean ± SE, estradiol 0.1 μM 78 ± 2 % of control (P < 0.05), 1 μM 76 ± 4 % of control (P < 0.05), 10 μM 67 ± 3 % of control, (P < 0.05)]. In addition, TGF-β1 production was significantly decreased [mean ± SE, estradiol 0.1 μM 96 ± 4 % of control (P = NS), 1 μM 84 ± 6 % of control (P < 0.05), 10 μM 70 ± 6 % of control, (P < 0.05)]. Conclusion: Estrogen increases vaginal SMC proliferation and inhibits tropoelastin and TGF-β1 production.
AB - Introduction and hypothesis: The aim of this study was to quantify the effects of estrogen on vaginal smooth muscle cell (SMC) tropoelastin and transforming growth factor (TGF)-β1 production. Methods: Primary SMC were incubated with estradiol, and cell proliferation was assessed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) assay at 48 h. Supernatants were collected and tropoelastin and TGF-β1 levels measured. Results: SMC proliferation was significantly increased by estradiol [relative cell number, mean ± standard error (SE), estradiol 0.1 μM 116 ± 19 % of control (P = NS), 1 μM 127 ± 13 % of control (P < 0.05), 10 μM 153 ± 26 % of control, (P < 0.05)]. Tropoelastin production was significantly decreased by estrogen [mean ± SE, estradiol 0.1 μM 78 ± 2 % of control (P < 0.05), 1 μM 76 ± 4 % of control (P < 0.05), 10 μM 67 ± 3 % of control, (P < 0.05)]. In addition, TGF-β1 production was significantly decreased [mean ± SE, estradiol 0.1 μM 96 ± 4 % of control (P = NS), 1 μM 84 ± 6 % of control (P < 0.05), 10 μM 70 ± 6 % of control, (P < 0.05)]. Conclusion: Estrogen increases vaginal SMC proliferation and inhibits tropoelastin and TGF-β1 production.
KW - Elastin
KW - Estrogen
KW - Transforming growth factor β1
KW - Vagina
UR - http://www.scopus.com/inward/record.url?scp=84877603554&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84877603554&partnerID=8YFLogxK
U2 - 10.1007/s00192-012-1828-6
DO - 10.1007/s00192-012-1828-6
M3 - Article
VL - 23
SP - 1791
EP - 1795
JO - International Urogynecology Journal and Pelvic Floor Dysfunction
JF - International Urogynecology Journal and Pelvic Floor Dysfunction
SN - 0937-3462
IS - 12
ER -