Estradiol-independent growth of a subline of MCF-7 human breast cancer cells in culture

H. Nawata, M. T. Chong, D. Bronzert, Marc E Lippman

Research output: Contribution to journalArticle

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Abstract

An estrogen-independent variant (R3) was selected by cloning wild type MCF-7 cells in soft agar in the presence of tamoxifen. R3 has a slightly slower growth rate under optimal growth conditions. Estradiol and tamoxifen have minimal effects on growth rate and thymidine incorporation as compared to wild type MCF-7 cells. R3 contains both cytoplasmic receptor and a receptor derived from crude nuclear extract with slightly higher numbers of cytosol receptor than wild type MCF-7. The same dissociation constant (Kd) and the same molecular weight (estimated by Sephadex chromatography) and sucrose density behavior for both cytoplasmic and nuclear estrogen receptor are seen in R3 and MCF-7. Estrogen receptor complexes were activated by salt and nucleotide and translocated to the nucleus equivalently in R3 and MCF-7. Relative binding affinities and extent of competition by estradiol and tamoxifen for cytoplasmic estrogen receptor are the same for MCF-7 and R3. Induction of progesterone receptor and nuclear estrogen receptor processing following treatment with estradiol in R3 is minimal compared with MCF-7. DNA affinity (as assessed by DNA cellulose chromatography) of estrogen receptor complexes from R3 abd MCF-7 is different. R3 appears to represent a cell with a defect expressed in receptor function apart from the initial hormone-binding step.

Original languageEnglish
Pages (from-to)6895-6902
Number of pages8
JournalJournal of Biological Chemistry
Volume256
Issue number13
StatePublished - Dec 1 1981
Externally publishedYes

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Cell culture
Estrogen Receptors
Estradiol
Tamoxifen
Cytoplasmic and Nuclear Receptors
Cell Culture Techniques
Cells
Breast Neoplasms
MCF-7 Cells
Growth
Chromatography
Estrogens
Progesterone Receptors
Complex Mixtures
Cytosol
Thymidine
Cloning
Agar
Sucrose
Organism Cloning

ASJC Scopus subject areas

  • Biochemistry

Cite this

Nawata, H., Chong, M. T., Bronzert, D., & Lippman, M. E. (1981). Estradiol-independent growth of a subline of MCF-7 human breast cancer cells in culture. Journal of Biological Chemistry, 256(13), 6895-6902.

Estradiol-independent growth of a subline of MCF-7 human breast cancer cells in culture. / Nawata, H.; Chong, M. T.; Bronzert, D.; Lippman, Marc E.

In: Journal of Biological Chemistry, Vol. 256, No. 13, 01.12.1981, p. 6895-6902.

Research output: Contribution to journalArticle

Nawata, H, Chong, MT, Bronzert, D & Lippman, ME 1981, 'Estradiol-independent growth of a subline of MCF-7 human breast cancer cells in culture', Journal of Biological Chemistry, vol. 256, no. 13, pp. 6895-6902.
Nawata, H. ; Chong, M. T. ; Bronzert, D. ; Lippman, Marc E. / Estradiol-independent growth of a subline of MCF-7 human breast cancer cells in culture. In: Journal of Biological Chemistry. 1981 ; Vol. 256, No. 13. pp. 6895-6902.
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