Estimation of Disk Membrane Lateral Pressure and Molecular Area of Rhodopsin by the Measurement of Its Orientation at the Nitrogen-Water Interface from an Ellipsometric Study

Christian Salesse, Daniel Ducharme, Roger M. Leblanc, François Boucher

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

The internal lateral pressure of a bilayer has been estimated by numerous investigators. Most of these measurements were made by using the monolayer technique. In our approach, the disk membrane lateral pressure was estimated by assuming that this value is equal to the surface pressure necessary to maintain the transmembrane orientation of rhodopsin. The orientation of rhodopsin at the nitrogen-water interface was determined by using ellipsometry, which can measure the thickness of the film. By examining surface pressure and ellipsometric isotherms of intact and partially hydrolyzed rhodopsin, we have determined that a lateral pressure of 38 mN/m is necessary to give rhodopsin its natural transmembrane orientation and that surface pressures exceeding 45 mN/m lead to the formation of multilayers in the disk membrane film. At 38 mN/m, pure rhodopsin is found to have a molecular area of 2300 Å2.

Original languageEnglish (US)
Pages (from-to)4567-4575
Number of pages9
JournalBiochemistry
Volume29
Issue number19
DOIs
StatePublished - May 1 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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