Estimation of acute blood loss in the anticoagulated rabbit model using 3 modalities of radio frequency energy ablation

Adam J. Ball, Raymond J. Leveillee, Michael F. Hoey, Vipul R. Patel, Sandy S. Kim

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Purpose: An anticoagulated animal model was tested to evaluate estimated acute blood loss (EABL) following tissue ablation with 3 modalities of radio frequency (RF) thermal energy. Materials and Methods: Four groups of randomly divided rabbits were established. Group 1 (3 control and 3 anticoagulated rabbits) underwent sham treatment (noRF), group 2 (2 control and 7 anticoagulated) received single probe dry RF (dRF) (475 KHz and 5 W for 2 minutes), group 3 (2 control and 7 anticoagulated) received single probe wet RF (wRF) (475 KHz with 14.6% hypertonic saline at 50 W for 40 seconds) and group 4 (3 control and 7 anticoagulated) was treated with vapor RF (vRF) (0.9% normal saline for 10 seconds). Oral warfarin sodium was the anticoagulant. Following a midline incision ablation was performed on the left kidney and liver. Pre-weighed gauze pads were used to collect EABL for a 5-minute observation period after needle probe removal. Temperature data were recorded from the right kidney using fiberoptic thermocouples. Lesions were grossly inspected and measured. Results: Anticoagulation resulted in super anticoagulated animals with an average prothrombin time of almost 140 seconds. EABL was the least from the ablated left kidney for vRF (50 mg), followed by wRF (260 mg), dRF (390 mg) and noRF (1,800 mg). EABL was the least from the liver for vRF (10 mg), followed by wRF (470 mg), dRF (1,260 mg) and noRF (2,680 mg). A greater percent of total ablative time at 10 mm was spent at greater than 50C during wRF and vRF. Measured ablative lesions size was largest following vRF ablation. Conclusions: The thermal coagulative effects of RF ablation resulted in less bleeding compared with controls in this orally anticoagulated animal model. The novel RF modality vRF is introduced.

Original languageEnglish
Pages (from-to)970-974
Number of pages5
JournalJournal of Urology
Volume170
Issue number3
DOIs
StatePublished - Sep 1 2003
Externally publishedYes

Fingerprint

Radio
Rabbits
Kidney
Animal Models
Hot Temperature
Liver
Prothrombin Time
Warfarin
Anticoagulants
Needles
Placebos
Observation
Hemorrhage
Temperature

Keywords

  • Anticoagulation
  • Hyperthermia, induced
  • Kidney
  • Rabbits
  • Warfarin

ASJC Scopus subject areas

  • Urology

Cite this

Estimation of acute blood loss in the anticoagulated rabbit model using 3 modalities of radio frequency energy ablation. / Ball, Adam J.; Leveillee, Raymond J.; Hoey, Michael F.; Patel, Vipul R.; Kim, Sandy S.

In: Journal of Urology, Vol. 170, No. 3, 01.09.2003, p. 970-974.

Research output: Contribution to journalArticle

Ball, Adam J. ; Leveillee, Raymond J. ; Hoey, Michael F. ; Patel, Vipul R. ; Kim, Sandy S. / Estimation of acute blood loss in the anticoagulated rabbit model using 3 modalities of radio frequency energy ablation. In: Journal of Urology. 2003 ; Vol. 170, No. 3. pp. 970-974.
@article{0db72583a34c4d67ae065afd0cc96e1a,
title = "Estimation of acute blood loss in the anticoagulated rabbit model using 3 modalities of radio frequency energy ablation",
abstract = "Purpose: An anticoagulated animal model was tested to evaluate estimated acute blood loss (EABL) following tissue ablation with 3 modalities of radio frequency (RF) thermal energy. Materials and Methods: Four groups of randomly divided rabbits were established. Group 1 (3 control and 3 anticoagulated rabbits) underwent sham treatment (noRF), group 2 (2 control and 7 anticoagulated) received single probe dry RF (dRF) (475 KHz and 5 W for 2 minutes), group 3 (2 control and 7 anticoagulated) received single probe wet RF (wRF) (475 KHz with 14.6{\%} hypertonic saline at 50 W for 40 seconds) and group 4 (3 control and 7 anticoagulated) was treated with vapor RF (vRF) (0.9{\%} normal saline for 10 seconds). Oral warfarin sodium was the anticoagulant. Following a midline incision ablation was performed on the left kidney and liver. Pre-weighed gauze pads were used to collect EABL for a 5-minute observation period after needle probe removal. Temperature data were recorded from the right kidney using fiberoptic thermocouples. Lesions were grossly inspected and measured. Results: Anticoagulation resulted in super anticoagulated animals with an average prothrombin time of almost 140 seconds. EABL was the least from the ablated left kidney for vRF (50 mg), followed by wRF (260 mg), dRF (390 mg) and noRF (1,800 mg). EABL was the least from the liver for vRF (10 mg), followed by wRF (470 mg), dRF (1,260 mg) and noRF (2,680 mg). A greater percent of total ablative time at 10 mm was spent at greater than 50C during wRF and vRF. Measured ablative lesions size was largest following vRF ablation. Conclusions: The thermal coagulative effects of RF ablation resulted in less bleeding compared with controls in this orally anticoagulated animal model. The novel RF modality vRF is introduced.",
keywords = "Anticoagulation, Hyperthermia, induced, Kidney, Rabbits, Warfarin",
author = "Ball, {Adam J.} and Leveillee, {Raymond J.} and Hoey, {Michael F.} and Patel, {Vipul R.} and Kim, {Sandy S.}",
year = "2003",
month = "9",
day = "1",
doi = "10.1097/01.ju.0000080048.75811.07",
language = "English",
volume = "170",
pages = "970--974",
journal = "Journal of Urology",
issn = "0022-5347",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - Estimation of acute blood loss in the anticoagulated rabbit model using 3 modalities of radio frequency energy ablation

AU - Ball, Adam J.

AU - Leveillee, Raymond J.

AU - Hoey, Michael F.

AU - Patel, Vipul R.

AU - Kim, Sandy S.

PY - 2003/9/1

Y1 - 2003/9/1

N2 - Purpose: An anticoagulated animal model was tested to evaluate estimated acute blood loss (EABL) following tissue ablation with 3 modalities of radio frequency (RF) thermal energy. Materials and Methods: Four groups of randomly divided rabbits were established. Group 1 (3 control and 3 anticoagulated rabbits) underwent sham treatment (noRF), group 2 (2 control and 7 anticoagulated) received single probe dry RF (dRF) (475 KHz and 5 W for 2 minutes), group 3 (2 control and 7 anticoagulated) received single probe wet RF (wRF) (475 KHz with 14.6% hypertonic saline at 50 W for 40 seconds) and group 4 (3 control and 7 anticoagulated) was treated with vapor RF (vRF) (0.9% normal saline for 10 seconds). Oral warfarin sodium was the anticoagulant. Following a midline incision ablation was performed on the left kidney and liver. Pre-weighed gauze pads were used to collect EABL for a 5-minute observation period after needle probe removal. Temperature data were recorded from the right kidney using fiberoptic thermocouples. Lesions were grossly inspected and measured. Results: Anticoagulation resulted in super anticoagulated animals with an average prothrombin time of almost 140 seconds. EABL was the least from the ablated left kidney for vRF (50 mg), followed by wRF (260 mg), dRF (390 mg) and noRF (1,800 mg). EABL was the least from the liver for vRF (10 mg), followed by wRF (470 mg), dRF (1,260 mg) and noRF (2,680 mg). A greater percent of total ablative time at 10 mm was spent at greater than 50C during wRF and vRF. Measured ablative lesions size was largest following vRF ablation. Conclusions: The thermal coagulative effects of RF ablation resulted in less bleeding compared with controls in this orally anticoagulated animal model. The novel RF modality vRF is introduced.

AB - Purpose: An anticoagulated animal model was tested to evaluate estimated acute blood loss (EABL) following tissue ablation with 3 modalities of radio frequency (RF) thermal energy. Materials and Methods: Four groups of randomly divided rabbits were established. Group 1 (3 control and 3 anticoagulated rabbits) underwent sham treatment (noRF), group 2 (2 control and 7 anticoagulated) received single probe dry RF (dRF) (475 KHz and 5 W for 2 minutes), group 3 (2 control and 7 anticoagulated) received single probe wet RF (wRF) (475 KHz with 14.6% hypertonic saline at 50 W for 40 seconds) and group 4 (3 control and 7 anticoagulated) was treated with vapor RF (vRF) (0.9% normal saline for 10 seconds). Oral warfarin sodium was the anticoagulant. Following a midline incision ablation was performed on the left kidney and liver. Pre-weighed gauze pads were used to collect EABL for a 5-minute observation period after needle probe removal. Temperature data were recorded from the right kidney using fiberoptic thermocouples. Lesions were grossly inspected and measured. Results: Anticoagulation resulted in super anticoagulated animals with an average prothrombin time of almost 140 seconds. EABL was the least from the ablated left kidney for vRF (50 mg), followed by wRF (260 mg), dRF (390 mg) and noRF (1,800 mg). EABL was the least from the liver for vRF (10 mg), followed by wRF (470 mg), dRF (1,260 mg) and noRF (2,680 mg). A greater percent of total ablative time at 10 mm was spent at greater than 50C during wRF and vRF. Measured ablative lesions size was largest following vRF ablation. Conclusions: The thermal coagulative effects of RF ablation resulted in less bleeding compared with controls in this orally anticoagulated animal model. The novel RF modality vRF is introduced.

KW - Anticoagulation

KW - Hyperthermia, induced

KW - Kidney

KW - Rabbits

KW - Warfarin

UR - http://www.scopus.com/inward/record.url?scp=0041736378&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0041736378&partnerID=8YFLogxK

U2 - 10.1097/01.ju.0000080048.75811.07

DO - 10.1097/01.ju.0000080048.75811.07

M3 - Article

C2 - 12913752

AN - SCOPUS:0041736378

VL - 170

SP - 970

EP - 974

JO - Journal of Urology

JF - Journal of Urology

SN - 0022-5347

IS - 3

ER -