Establishment of two human prostate cancer cell lines derived from a single bone metastasis

Nora M. Navone, Matilde Olive, Mustafa Ozen, Rodney Davis, Patricia Troncoso, Shi Ming Tu, Dennis Johnston, Alan Pollack, Sen Pathak, Andrew C. Von Eschenbach, Christopher J. Logothetis

Research output: Contribution to journalArticle

189 Citations (Scopus)

Abstract

Human prostate cancer cell lines are particularly difficult to establish, and most existing cell lines do not exhibit features commonly seen in human prostate cancer. Most available models either grow only in vivo as xenografts or are androgen insensitive and fail to express prostate-specific antigen (PSA). The lack of functionally relevant model systems of advanced prostate cancer has limited prostate cancer research and therapy development. Of 30 processed samples derived from patients with prostate cancer, we established two cell lines (MDA PCa 2a and MDA PCa 2b) that express PSA and androgen receptor, grow in vitro, and are androgen sensitive. Cells from these lines produced tumors in nude mice when injected either s.c. or orthotopically (intraprostatic). Both cell lines were established from a bone metastasis of a patient whose cancer was exhibiting androgen-independent growth. Although both were derived from two samples of the same specimen, they have different genetic features (as assessed by karyotype analysis) and different phenotypes (e.g., morphology and growth rate). It is likely that they are distinct clones isolated by the use of different culture procedures and reflect the genetic heterogeneity of the tumor. These new cell lines are the first available derived from a bone metastasis of an androgen-independent prostatic adenocarcinoma that grow both in vivo and in vitro and have retained PSA expression and androgen sensitivity. They therefore constitute important model systems to address critical questions related to the androgen-independent growth of human prostate cancer and to the complex process of bone metastasis.

Original languageEnglish
Pages (from-to)2493-2500
Number of pages8
JournalClinical Cancer Research
Volume3
Issue number12 I
StatePublished - Dec 1 1997
Externally publishedYes

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Androgens
Prostatic Neoplasms
Neoplasm Metastasis
Bone and Bones
Cell Line
Prostate-Specific Antigen
Growth
Antigen Receptors
Genetic Heterogeneity
Androgen Receptors
Tumor Cell Line
Karyotype
Heterografts
Nude Mice
Neoplasms
Adenocarcinoma
Clone Cells
Phenotype
Research
In Vitro Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Navone, N. M., Olive, M., Ozen, M., Davis, R., Troncoso, P., Tu, S. M., ... Logothetis, C. J. (1997). Establishment of two human prostate cancer cell lines derived from a single bone metastasis. Clinical Cancer Research, 3(12 I), 2493-2500.

Establishment of two human prostate cancer cell lines derived from a single bone metastasis. / Navone, Nora M.; Olive, Matilde; Ozen, Mustafa; Davis, Rodney; Troncoso, Patricia; Tu, Shi Ming; Johnston, Dennis; Pollack, Alan; Pathak, Sen; Von Eschenbach, Andrew C.; Logothetis, Christopher J.

In: Clinical Cancer Research, Vol. 3, No. 12 I, 01.12.1997, p. 2493-2500.

Research output: Contribution to journalArticle

Navone, NM, Olive, M, Ozen, M, Davis, R, Troncoso, P, Tu, SM, Johnston, D, Pollack, A, Pathak, S, Von Eschenbach, AC & Logothetis, CJ 1997, 'Establishment of two human prostate cancer cell lines derived from a single bone metastasis', Clinical Cancer Research, vol. 3, no. 12 I, pp. 2493-2500.
Navone NM, Olive M, Ozen M, Davis R, Troncoso P, Tu SM et al. Establishment of two human prostate cancer cell lines derived from a single bone metastasis. Clinical Cancer Research. 1997 Dec 1;3(12 I):2493-2500.
Navone, Nora M. ; Olive, Matilde ; Ozen, Mustafa ; Davis, Rodney ; Troncoso, Patricia ; Tu, Shi Ming ; Johnston, Dennis ; Pollack, Alan ; Pathak, Sen ; Von Eschenbach, Andrew C. ; Logothetis, Christopher J. / Establishment of two human prostate cancer cell lines derived from a single bone metastasis. In: Clinical Cancer Research. 1997 ; Vol. 3, No. 12 I. pp. 2493-2500.
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