Establishment of primary cell cultures from human and canine parathyroid gland explants

F. J. Hornicek, E. Reiss, Theodore Malinin, G. I. Malinin

Research output: Contribution to journalArticle

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Abstract

Primary parathyroid cell cultures were established from 22 canine and 20 human parathyroid glands explanted into Connaught Medical Research Laboratory-1415 (CMRL-1415) medium containing fetal calf serum (FCS). Initiation of cellular outgrowth and the rate of cellular propagation through the first subcultures were evaluated in various media. These included Roswell Park Memorial Institute-1640 (RPMI-1640), minimal essential medium (MEM), medium-199 (M-199) and Coon's modified Ham's F-12 (F12) and CMRL-1415 media. Only CMRL-1415 medium supplemented with FCS invariably supported the vigorous rate of cellular growth as determined by the number of cells in cultures, their viability, parathyroid hormone (PTH) levels in the growth media and the number of proliferating cells. Propagation of parathyroid cells in vitro was limited to five passages. Cessation of cell division was always coincidental with the emergence of numerous, non-dividing binucleated cells. These experiments demonstrate that primary parathyroid cell cultures from canine and human parathyroid gland explants can be readily established in CMRL-1415 medium containing FCS.

Original languageEnglish
Pages (from-to)157-165
Number of pages9
JournalBone and Mineral
Volume4
Issue number2
StatePublished - Jan 1 1988

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Parathyroid Glands
Primary Cell Culture
Research laboratories
Cell culture
Canidae
Biomedical Research
Serum
Growth
Parathyroid Hormone
Cell Division
Cell Culture Techniques
Cell Count
Cells
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Surgery

Cite this

Hornicek, F. J., Reiss, E., Malinin, T., & Malinin, G. I. (1988). Establishment of primary cell cultures from human and canine parathyroid gland explants. Bone and Mineral, 4(2), 157-165.

Establishment of primary cell cultures from human and canine parathyroid gland explants. / Hornicek, F. J.; Reiss, E.; Malinin, Theodore; Malinin, G. I.

In: Bone and Mineral, Vol. 4, No. 2, 01.01.1988, p. 157-165.

Research output: Contribution to journalArticle

Hornicek, FJ, Reiss, E, Malinin, T & Malinin, GI 1988, 'Establishment of primary cell cultures from human and canine parathyroid gland explants', Bone and Mineral, vol. 4, no. 2, pp. 157-165.
Hornicek FJ, Reiss E, Malinin T, Malinin GI. Establishment of primary cell cultures from human and canine parathyroid gland explants. Bone and Mineral. 1988 Jan 1;4(2):157-165.
Hornicek, F. J. ; Reiss, E. ; Malinin, Theodore ; Malinin, G. I. / Establishment of primary cell cultures from human and canine parathyroid gland explants. In: Bone and Mineral. 1988 ; Vol. 4, No. 2. pp. 157-165.
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