Establishment of permanent cell lines by Epstein-Barr virus transformation.

J. Gilbert

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

This basic protocol presents Epstein-Barr virus (EBV) transformation of B cells to create a permanent cell line to provide cells for genetic and DNA analysis. Virus stocks are prepared from cultures of an infected marmoset cell line in the first support protocol. Transformed cells may be analyzed immediately or frozen for future use as described in the second support protocol.

Original languageEnglish (US)
Pages (from-to)Appendix 3H
JournalCurrent protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]
VolumeAppendix 3
StatePublished - May 2001

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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