Establishment of permanent cell lines by Epstein-Barr virus transformation.

John Gilbert

Research output: Contribution to journal › Article

1 Citation (Scopus)

Abstract

This basic protocol presents Epstein-Barr virus (EBV) transformation of B cells to create a permanent cell line to provide cells for genetic and DNA analysis. Virus stocks are prepared from cultures of an infected marmoset cell line in the first support protocol. Transformed cells may be analyzed immediately or frozen for future use as described in the second support protocol.

Original languageEnglish
JournalCurrent protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]
VolumeAppendix 3
StatePublished - May 1 2001
Externally publishedYes

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Human Herpesvirus 4
Cell Line
Callithrix
B-Lymphocytes
Viruses
DNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Establishment of permanent cell lines by Epstein-Barr virus transformation. / Gilbert, John.

In: Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.], Vol. Appendix 3, 01.05.2001.

Research output: Contribution to journal › Article

Gilbert, J 2001, 'Establishment of permanent cell lines by Epstein-Barr virus transformation.', Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.], vol. Appendix 3.
Gilbert J. Establishment of permanent cell lines by Epstein-Barr virus transformation. Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]. 2001 May 1;Appendix 3.
Gilbert, John. / Establishment of permanent cell lines by Epstein-Barr virus transformation. In: Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.]. 2001 ; Vol. Appendix 3.
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