Abstract
This basic protocol presents Epstein-Barr virus (EBV) transformation of B cells to create a permanent cell line to provide cells for genetic and DNA analysis. Virus stocks are prepared from cultures of an infected marmoset cell line in the first support protocol. Transformed cells may be analyzed immediately or frozen for future use as described in the second support protocol.
| Original language | English (US) |
|---|---|
| Pages (from-to) | Appendix 3H |
| Journal | Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.] |
| Volume | Appendix 3 |
| State | Published - May 2001 |
ASJC Scopus subject areas
- Genetics
- Genetics(clinical)
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Cite this
Gilbert, J. (2001). Establishment of permanent cell lines by Epstein-Barr virus transformation. Current protocols in human genetics / editorial board, Jonathan L. Haines ... [et al.], Appendix 3, Appendix 3H.