Establishment of human pancreatic ductal cells in a long-term culture

Alexis B. Ulrich, Bruno M. Schmied, Hosei Matsuzaki, Tarek El-Metwally, Mary P. Moyer, Camillo Ricordi, Thomas E. Adrian, Surinder K. Batra, Parviz M. Pour

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Cultivation and preservation of human pancreatic ductal cells have remained a challenge. With a defined culture medium and refinement of culturing techniques, we have been able to maintain human pancreatic ductal cells without any genetic manipulation in culture for more than 16 months. Freshly isolated ductal fragments were placed on a rocker in M3:5 medium free of collagen for 14 days to remove fibroblasts and endocrine cells before allowing them to attach. The cells produced an excessive amount of mucin and expressed the duct specific cytokeratins (CK) 7 and 19, DU-PAN2, CA19-9, carbonic anhydrase II (CA II), and secretin receptors. During the course of the culture, however, the cells gradually lost the expression of CA II, secretin receptors, DU-PAN2, and CA 19-9 and assumed an undifferentiated pheno-type, which showed an upregulation of transforming growth factor α (TGFα) and epidermal growth factor receptor (EGFR), an increase in the expression of Ki-67, and an increased binding to Phaseolus vulgaris leucoagglutinin (PHA-L) and tomato lectin. These ductal cells present a useful source with which to study physiologic aspects of ductal cells including differentiation.

Original languageEnglish (US)
Pages (from-to)358-368
Number of pages11
JournalPancreas
Volume21
Issue number4
DOIs
StatePublished - Nov 11 2000

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Keywords

  • Cell culture
  • Differentiation
  • Normal human pancreatic duct cells

ASJC Scopus subject areas

  • Gastroenterology
  • Endocrinology

Cite this

Ulrich, A. B., Schmied, B. M., Matsuzaki, H., El-Metwally, T., Moyer, M. P., Ricordi, C., Adrian, T. E., Batra, S. K., & Pour, P. M. (2000). Establishment of human pancreatic ductal cells in a long-term culture. Pancreas, 21(4), 358-368. https://doi.org/10.1097/00006676-200011000-00006