Establishment and characterization of a primary effusion (body cavity- based) lymphoma cell line (BC-3) harboring Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) in the absence of Epstein-Barr virus

Leandros Arvanitakis, Enrique A Mesri, Roland G. Nador, Jonathan W. Said, Adam S. Asch, Daniel M. Knowles, Ethel Cesarman

Research output: Contribution to journalArticle

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Abstract

The recently identified Kaposi's sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus 8 (HHV-8), has been found to be consistently associated with an unusual subset of acquired immunodeficiency syndrome- related lymphomas, the so-called body cavity-based lymphomas (BCBL) or primary effusion lymphomas (PEL). These lymphomas are characterized by a unique spectrum of morphologic and molecular characteristics, and grow as lymphomatous effusions without an identifiable contiguous tumor mass. Until now, efforts to delineate the role of KSHV in the pathogenesis of PELs have been hampered by the lack of appropriate model systems and the concomitant presence of Epstein-Barr virus (EBV) in nearly all cases examined, and in all previously established cell lines. We now report the establishment and characterization of a novel PEL cell line, BC-3, which is KSHV+ by polymerase chain reaction (PCR) but EBV- as assessed by a variety of methods including PCR for EBER, EBNA-2, and EBNA-3C. This cell line was established from a lymphomatous effusion obtained from an HIV- patient, and has immunophenotypic and molecular features consistent with the diagnosis of PEL, including an indeterminate immunophenotype with a B-cell immunogenotype and lack of c-myc proto-oncogene rearrangements. Pulsed-field gel electrophoresis shows an intact KSHV genome of about 170 kb both in the cell line and in the viral isolate, whereas herpesvirus-like capsids are visible by electron microscopy. Consequently, the BC-3 cell line represents an invaluable tool as a source of KSHV, for both the evaluation of the pathogenic potential of this virus and the mechanistic characterization of its role in the development of Kaposi's sarcoma and malignant lymphoma.

Original languageEnglish
Pages (from-to)2648-2654
Number of pages7
JournalBlood
Volume88
Issue number7
StatePublished - Oct 1 1996
Externally publishedYes

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Human Herpesvirus 8
Human Herpesvirus 4
Viruses
Lymphoma
Primary Effusion Lymphoma
Cells
Cell Line
Polymerase chain reaction
Polymerase Chain Reaction
myc Genes
Kaposi's Sarcoma
Capsid
Herpesviridae
Pulsed Field Gel Electrophoresis
Electrophoresis
Electron microscopy
Tumors
Electron Microscopy
Acquired Immunodeficiency Syndrome
B-Lymphocytes

ASJC Scopus subject areas

  • Hematology

Cite this

Establishment and characterization of a primary effusion (body cavity- based) lymphoma cell line (BC-3) harboring Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) in the absence of Epstein-Barr virus. / Arvanitakis, Leandros; Mesri, Enrique A; Nador, Roland G.; Said, Jonathan W.; Asch, Adam S.; Knowles, Daniel M.; Cesarman, Ethel.

In: Blood, Vol. 88, No. 7, 01.10.1996, p. 2648-2654.

Research output: Contribution to journalArticle

Arvanitakis, Leandros ; Mesri, Enrique A ; Nador, Roland G. ; Said, Jonathan W. ; Asch, Adam S. ; Knowles, Daniel M. ; Cesarman, Ethel. / Establishment and characterization of a primary effusion (body cavity- based) lymphoma cell line (BC-3) harboring Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) in the absence of Epstein-Barr virus. In: Blood. 1996 ; Vol. 88, No. 7. pp. 2648-2654.
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abstract = "The recently identified Kaposi's sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus 8 (HHV-8), has been found to be consistently associated with an unusual subset of acquired immunodeficiency syndrome- related lymphomas, the so-called body cavity-based lymphomas (BCBL) or primary effusion lymphomas (PEL). These lymphomas are characterized by a unique spectrum of morphologic and molecular characteristics, and grow as lymphomatous effusions without an identifiable contiguous tumor mass. Until now, efforts to delineate the role of KSHV in the pathogenesis of PELs have been hampered by the lack of appropriate model systems and the concomitant presence of Epstein-Barr virus (EBV) in nearly all cases examined, and in all previously established cell lines. We now report the establishment and characterization of a novel PEL cell line, BC-3, which is KSHV+ by polymerase chain reaction (PCR) but EBV- as assessed by a variety of methods including PCR for EBER, EBNA-2, and EBNA-3C. This cell line was established from a lymphomatous effusion obtained from an HIV- patient, and has immunophenotypic and molecular features consistent with the diagnosis of PEL, including an indeterminate immunophenotype with a B-cell immunogenotype and lack of c-myc proto-oncogene rearrangements. Pulsed-field gel electrophoresis shows an intact KSHV genome of about 170 kb both in the cell line and in the viral isolate, whereas herpesvirus-like capsids are visible by electron microscopy. Consequently, the BC-3 cell line represents an invaluable tool as a source of KSHV, for both the evaluation of the pathogenic potential of this virus and the mechanistic characterization of its role in the development of Kaposi's sarcoma and malignant lymphoma.",
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AU - Mesri, Enrique A

AU - Nador, Roland G.

AU - Said, Jonathan W.

AU - Asch, Adam S.

AU - Knowles, Daniel M.

AU - Cesarman, Ethel

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