Establishment and characterization of a novel chordoma cell line: CH22

Xianzhe Liu, Gunnlager Petur Nielsen, Andrew Rosenberg, Peter R. Waterman, Wen Yang, Edwin Choy, Slim Sassi, Shuhua Yang, David C. Harmon, Cao Yang, Joseph H. Schwab, Eisuke Kobayashi, Henry J. Mankin, Ramnik Xavier, Ralph Weissleder, Zhenfeng Duan, Francis J. Hornicek

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Chordoma is a rare primary malignant bone tumor and there exist only a few established human chordoma cell lines. The scarcity of robust chordoma cell lines has limited the ability to study this tumor. In this report, we describe the establishment of a novel chordoma cell line and characterize its in vitro and in vivo behaviors. The tumor tissue was isolated from a patient with recurrent chordoma of the sacrum. After 6 months in culture, the chordoma cell line, referred here as CH22, was established. Microscopic analysis of two-dimensional culture confirmed that the CH22 cells exhibited a typical vacuolated cytoplasm similar to the well-established chordoma cell line U-CH1. Electron microscopy showed cohesive cells with numerous surface filopodia, pockets of glycogen and aggregates of intermediate tonofilaments in cytoplasm. Three-dimensional culture revealed that the CH22 cells could grow and form clusters by day 8. The MTT assays demonstrated that, compared with sensitive osteosarcoma cell lines, CH22 cells were relatively resistant to conventional chemotherapeutic drugs. Western blotting and immunofluorescence analysis confirmed that the CH22 cells expressed brachyury, vimentin, and cytokeratin. Finally, histological analysis of CH22 xenograft tumor tissues demonstrated the appearance of physaliphorous cells and positive staining of brachyury, cytokeratin, and S100. By CT and MRI, imaging xenografts showed the typical appearances seen in human chordomas. These findings suggest that the established novel human chordoma cell line CH22 and its tumorigenecity in SCID nude mice may serve as an important model for studying chordoma cell biology and the development of new therapeutic modalities. ©

Original languageEnglish
Pages (from-to)1666-1673
Number of pages8
JournalJournal of Orthopaedic Research
Volume30
Issue number10
DOIs
StatePublished - Oct 1 2012
Externally publishedYes

Fingerprint

Chordoma
Cell Line
Keratins
Heterografts
Neoplasms
Cytoplasm
Sacrum
Pseudopodia
Aptitude
SCID Mice
Intermediate Filaments
Vimentin
Osteosarcoma
Glycogen
Nude Mice
Fluorescent Antibody Technique
Cell Biology
Electron Microscopy
Western Blotting
Staining and Labeling

Keywords

  • 3D culture
  • chemotherapy
  • chordoma
  • tumorigenicity
  • xenograft

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Liu, X., Nielsen, G. P., Rosenberg, A., Waterman, P. R., Yang, W., Choy, E., ... Hornicek, F. J. (2012). Establishment and characterization of a novel chordoma cell line: CH22. Journal of Orthopaedic Research, 30(10), 1666-1673. https://doi.org/10.1002/jor.22113

Establishment and characterization of a novel chordoma cell line : CH22. / Liu, Xianzhe; Nielsen, Gunnlager Petur; Rosenberg, Andrew; Waterman, Peter R.; Yang, Wen; Choy, Edwin; Sassi, Slim; Yang, Shuhua; Harmon, David C.; Yang, Cao; Schwab, Joseph H.; Kobayashi, Eisuke; Mankin, Henry J.; Xavier, Ramnik; Weissleder, Ralph; Duan, Zhenfeng; Hornicek, Francis J.

In: Journal of Orthopaedic Research, Vol. 30, No. 10, 01.10.2012, p. 1666-1673.

Research output: Contribution to journalArticle

Liu, X, Nielsen, GP, Rosenberg, A, Waterman, PR, Yang, W, Choy, E, Sassi, S, Yang, S, Harmon, DC, Yang, C, Schwab, JH, Kobayashi, E, Mankin, HJ, Xavier, R, Weissleder, R, Duan, Z & Hornicek, FJ 2012, 'Establishment and characterization of a novel chordoma cell line: CH22', Journal of Orthopaedic Research, vol. 30, no. 10, pp. 1666-1673. https://doi.org/10.1002/jor.22113
Liu, Xianzhe ; Nielsen, Gunnlager Petur ; Rosenberg, Andrew ; Waterman, Peter R. ; Yang, Wen ; Choy, Edwin ; Sassi, Slim ; Yang, Shuhua ; Harmon, David C. ; Yang, Cao ; Schwab, Joseph H. ; Kobayashi, Eisuke ; Mankin, Henry J. ; Xavier, Ramnik ; Weissleder, Ralph ; Duan, Zhenfeng ; Hornicek, Francis J. / Establishment and characterization of a novel chordoma cell line : CH22. In: Journal of Orthopaedic Research. 2012 ; Vol. 30, No. 10. pp. 1666-1673.
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