Establishment and characterization of a cell line derived from a spontaneous murinelung carcinoma (M109)

Richard Schultz, Phillip Ruiz, Michael Chirigos, Ursula Heine, Walter Nelson-Rees

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8 Scopus citations


The Madison lung (M109) tumor cell line, initiated from a "spontaneous", anaplastic murine lung carcinoma, has been propagated continuously in vitro for more than 300 cell generations. Cytogenetic analysis revealed a mouse karyotype with a mode of 78 chromosomes (2n=40). Three distinct marker chromosomes were identified by trypsin-giemsa banding. The cells piled up in culture and had a short generation time and high plating efficiency. Electron microscopy revealed highly undifferentiated cells with little rough endoplasmic reticulum, an abundance of free polysomes, the presence of few and often oddshaped mitochondria, lipid bodies and phagocytic vacuoles. Virus particles of the C-type were found frequently. The subcutaneous transplantation of M109 cultured cells at a relatively low cell inoculum produced highly metastatic tumors in syngeneic BALB/c mice.

Original languageEnglish
Pages (from-to)223-231
Number of pages9
JournalIn Vitro
Issue number4
StatePublished - Apr 1 1977
Externally publishedYes



  • C-type virus
  • cell culture
  • lung carcinoma

ASJC Scopus subject areas

  • Developmental Biology
  • Clinical Biochemistry
  • Cell Biology

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