Essential role for synaptopodin in dendritic spine plasticity of the developing hippocampus

Xiao Lei Zhang, Beatrice Pöschel, Christian H Faul, Chirag Upreti, Patric K. Stanton, Peter Mundel

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Dendritic spines are a major substrate of brain plasticity. Although many studies have focused on Ca 2+ /calmodulin-dependent protein kinase II (CaMKII)-mediated regulation of spine dynamics and synaptic function in adult brain, much less is know about protein kinase A (PKA)-dependent regulation of spine shape dynamics during postnatal brain development. Synaptopodin is a dendritic spine associated modulator of actin dynamics and a substrate of PKA. Here we show that NMDA and cAMP-induced dendritic spine expansion is impaired in hippocampal slices from 15-and 21-d-old synaptopodin-deficient mice. We further show that synaptopodin is required for full expression of PKA-dependent hippocampal long-term potentiation in 15-and 21-d-old, but not adult, mice. PKA-induced cAMP response element-binding phosphorylation is normal in the hippocampus of synaptopodin-deficient mice, suggesting that synaptopodin functions independently of cAMP response element-binding. Our results identify synaptopodin as a substrate of PKA in hippocampal neurons and point to an essential role for synaptopodin in activity-dependent regulation of dendritic spine dynamics and synaptic plasticity in postnatal brain development.

Original languageEnglish
Pages (from-to)12510-12518
Number of pages9
JournalJournal of Neuroscience
Volume33
Issue number30
DOIs
StatePublished - Jul 30 2013

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Dendritic Spines
Cyclic AMP-Dependent Protein Kinases
Hippocampus
Brain
Response Elements
Spine
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Calcium-Calmodulin-Dependent Protein Kinases
Neuronal Plasticity
Long-Term Potentiation
N-Methylaspartate
Actins
Phosphorylation
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Zhang, X. L., Pöschel, B., Faul, C. H., Upreti, C., Stanton, P. K., & Mundel, P. (2013). Essential role for synaptopodin in dendritic spine plasticity of the developing hippocampus. Journal of Neuroscience, 33(30), 12510-12518. https://doi.org/10.1523/JNEUROSCI.2983-12.2013

Essential role for synaptopodin in dendritic spine plasticity of the developing hippocampus. / Zhang, Xiao Lei; Pöschel, Beatrice; Faul, Christian H; Upreti, Chirag; Stanton, Patric K.; Mundel, Peter.

In: Journal of Neuroscience, Vol. 33, No. 30, 30.07.2013, p. 12510-12518.

Research output: Contribution to journalArticle

Zhang, XL, Pöschel, B, Faul, CH, Upreti, C, Stanton, PK & Mundel, P 2013, 'Essential role for synaptopodin in dendritic spine plasticity of the developing hippocampus', Journal of Neuroscience, vol. 33, no. 30, pp. 12510-12518. https://doi.org/10.1523/JNEUROSCI.2983-12.2013
Zhang, Xiao Lei ; Pöschel, Beatrice ; Faul, Christian H ; Upreti, Chirag ; Stanton, Patric K. ; Mundel, Peter. / Essential role for synaptopodin in dendritic spine plasticity of the developing hippocampus. In: Journal of Neuroscience. 2013 ; Vol. 33, No. 30. pp. 12510-12518.
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