Escherichia coli RNase M, is a multiply altered form of RNase I

Subbayan Pochi, Murray P Deutscher

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

RNase M, an enzyme previously purified to homogeneity from Escherichia coli, was suggested to be the RNase responsible for mRNA degradation in this bacterium. Although related to the endoribonuclease, RNase I, its distinct properties led to the conclusion that RNase M was a second, low molecular mass, broad specificity endoribonuclease present in E. coli. However, based on sequence analysis, southern hybridization, and enzyme activity, we show that RNase M is, in fact, a multiply altered form of RNase I. In addition to three amino acid substitutions that confer the properties of RNase M on the mutated RNase I, the protein is synthesized from an rna gene that contains a UGA nonsense codon at position 5, apparently as a result of a low level of readthrough. We also suggest that RNase M is just one of several previously described endoribonuclease activities that are actually manifestations of RNase I.

Original languageEnglish
Pages (from-to)1702-1707
Number of pages6
JournalRNA
Volume7
Issue number12
StatePublished - Dec 1 2001

Fingerprint

Pancreatic Ribonuclease
Endoribonucleases
Escherichia coli
Terminator Codon
Nonsense Codon
RNA Stability
Enzymes
Amino Acid Substitution
Ribonucleases
Sequence Analysis
ribonuclease M
RNA
Bacteria
Genes
Proteins

Keywords

  • Endoribonuclease
  • RNA degradation

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Pochi, S., & Deutscher, M. P. (2001). Escherichia coli RNase M, is a multiply altered form of RNase I. RNA, 7(12), 1702-1707.

Escherichia coli RNase M, is a multiply altered form of RNase I. / Pochi, Subbayan; Deutscher, Murray P.

In: RNA, Vol. 7, No. 12, 01.12.2001, p. 1702-1707.

Research output: Contribution to journalArticle

Pochi, S & Deutscher, MP 2001, 'Escherichia coli RNase M, is a multiply altered form of RNase I', RNA, vol. 7, no. 12, pp. 1702-1707.
Pochi S, Deutscher MP. Escherichia coli RNase M, is a multiply altered form of RNase I. RNA. 2001 Dec 1;7(12):1702-1707.
Pochi, Subbayan ; Deutscher, Murray P. / Escherichia coli RNase M, is a multiply altered form of RNase I. In: RNA. 2001 ; Vol. 7, No. 12. pp. 1702-1707.
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