TY - JOUR
T1 - Escherichia coli RNase D
T2 - Sequencing of the rnd structural gene and purification of the overexpressed protein
AU - Zhang, Jiren
AU - Deutscher, Murray P.
N1 - Funding Information:
This work was supported by Grant GM16317 from the National Institutes of Health. This is paper 52 in the series, "Reactions at the 3' Terminus of tRNA". The previous paper in this series is ref. 28.
PY - 1988/7/25
Y1 - 1988/7/25
N2 - We have determined the nucleotide sequence of a 1.4-kb-pair fragment of the E. coli chromosome that carries the complete rnd gene encoding RNase D, a putative tRNA processing enzyme. The coding region of rnd extends for a total of 1128 nucleotides beginning at an initiator UUG codon and terminating at a UAA codon, and encodes a 375-amino acid polypeptide of 42,679 daltons, consistent with the known size of RNase D. A rapid purification procedure was developed for isolation of RNase D from strains overexpressing the enzyme. The N-terminal sequence and the amino acid composition of the homogenous protein were in excellent agreement with those derived from the sequence of the rnd gene.
AB - We have determined the nucleotide sequence of a 1.4-kb-pair fragment of the E. coli chromosome that carries the complete rnd gene encoding RNase D, a putative tRNA processing enzyme. The coding region of rnd extends for a total of 1128 nucleotides beginning at an initiator UUG codon and terminating at a UAA codon, and encodes a 375-amino acid polypeptide of 42,679 daltons, consistent with the known size of RNase D. A rapid purification procedure was developed for isolation of RNase D from strains overexpressing the enzyme. The N-terminal sequence and the amino acid composition of the homogenous protein were in excellent agreement with those derived from the sequence of the rnd gene.
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U2 - 10.1093/nar/16.14.6265
DO - 10.1093/nar/16.14.6265
M3 - Article
C2 - 3041371
AN - SCOPUS:0023705439
VL - 16
SP - 6265
EP - 6278
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 14
ER -