Abstract
We have determined the nucleotide sequence of a 1.4-kb-pair fragment of the E. coli chromosome that carries the complete rnd gene encoding RNase D, a putative tRNA processing enzyme. The coding region of rnd extends for a total of 1128 nucleotides beginning at an initiator UUG codon and terminating at a UAA codon, and encodes a 375-amino acid polypeptide of 42,679 daltons, consistent with the known size of RNase D. A rapid purification procedure was developed for isolation of RNase D from strains overexpressing the enzyme. The N-terminal sequence and the amino acid composition of the homogenous protein were in excellent agreement with those derived from the sequence of the rnd gene.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 6265-6278 |
| Number of pages | 14 |
| Journal | Nucleic acids research |
| Volume | 16 |
| Issue number | 14 |
| DOIs | |
| State | Published - Jul 25 1988 |
ASJC Scopus subject areas
- Genetics
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Zhang, J., & Deutscher, M. P. (1988). Escherichia coli RNase D: Sequencing of the rnd structural gene and purification of the overexpressed protein. Nucleic acids research, 16(14), 6265-6278. https://doi.org/10.1093/nar/16.14.6265