Escherichia coli rna gene encoding RNase I: Cloning overexpression, subcellular distribution of the enzyme, and use of an rna deletion to identify additional RNases

L. Zhu, T. Gangopadhyay, K. P. Padmanabha, Murray P Deutscher

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The cloning and overexpression of the Escherichia coli rna gene encoding RNase I are described. Only a single copy of the rna gene is present on the E. coli chromosome. Although cells with as much as a 100-fold increase in RNase I activity were constructed, little effect on cell growth was observed. Overexpressed RNase I was found in the periplasmic space to the same degree (~85%) as wild-type enzyme, suggesting no limitation in RNase I transport. The rna clone was used to identify a deletion strain totally lacking the rna gene. The normal growth of this strain showed that RNase I is not essential for cell viability. Extracts from the RNase I deletion strain still retained a low level of RNase activity in the presence of EDTA, conclusively demonstrating the existence of additional EDTA-active RNases in E. coli. The possibility of a RNase I inhibitor is also discussed.

Original languageEnglish
Pages (from-to)3146-3151
Number of pages6
JournalJournal of Bacteriology
Volume172
Issue number6
StatePublished - Jun 15 1990
Externally publishedYes

Fingerprint

Pancreatic Ribonuclease
Ribonucleases
Organism Cloning
RNA
Escherichia coli
Enzymes
Genes
Edetic Acid
Periplasm
Growth
Cell Survival
Clone Cells
Chromosomes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Escherichia coli rna gene encoding RNase I : Cloning overexpression, subcellular distribution of the enzyme, and use of an rna deletion to identify additional RNases. / Zhu, L.; Gangopadhyay, T.; Padmanabha, K. P.; Deutscher, Murray P.

In: Journal of Bacteriology, Vol. 172, No. 6, 15.06.1990, p. 3146-3151.

Research output: Contribution to journalArticle

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