Escherichia coli orfE (upstream of pyrE) encodes RNase PH

K. A. Ost, Murray P Deutscher

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

RNase PH from extracts of Escherichia coli was purified to homogeneity and subjected to NH2-terminal sequencing. Comparison of this sequence with all open reading frames in the GenBank data base revealed at least 95% identity to an unidentified open reading frame (orfE) upstream of pyrE at 81.7 min on the E. coli chromosome. Clones of orfE overexpress RNase PH activity, verifying that orfE encodes this ribonuclease. We suggest that orfE be renamed rph.

Original languageEnglish
Pages (from-to)5589-5591
Number of pages3
JournalJournal of Bacteriology
Volume173
Issue number17
StatePublished - Oct 18 1991
Externally publishedYes

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Open Reading Frames
Escherichia coli
Nucleic Acid Databases
Ribonucleases
ribonuclease PH
Clone Cells
Chromosomes
Databases

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Ost, K. A., & Deutscher, M. P. (1991). Escherichia coli orfE (upstream of pyrE) encodes RNase PH. Journal of Bacteriology, 173(17), 5589-5591.

Escherichia coli orfE (upstream of pyrE) encodes RNase PH. / Ost, K. A.; Deutscher, Murray P.

In: Journal of Bacteriology, Vol. 173, No. 17, 18.10.1991, p. 5589-5591.

Research output: Contribution to journalArticle

Ost, KA & Deutscher, MP 1991, 'Escherichia coli orfE (upstream of pyrE) encodes RNase PH', Journal of Bacteriology, vol. 173, no. 17, pp. 5589-5591.
Ost KA, Deutscher MP. Escherichia coli orfE (upstream of pyrE) encodes RNase PH. Journal of Bacteriology. 1991 Oct 18;173(17):5589-5591.
Ost, K. A. ; Deutscher, Murray P. / Escherichia coli orfE (upstream of pyrE) encodes RNase PH. In: Journal of Bacteriology. 1991 ; Vol. 173, No. 17. pp. 5589-5591.
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