Escherichia coli orfE (upstream of pyrE) encodes RNase PH

K. A. Ost, M. P. Deutscher

Research output: Contribution to journalComment/debatepeer-review

13 Scopus citations


RNase PH from extracts of Escherichia coli was purified to homogeneity and subjected to NH2-terminal sequencing. Comparison of this sequence with all open reading frames in the GenBank data base revealed at least 95% identity to an unidentified open reading frame (orfE) upstream of pyrE at 81.7 min on the E. coli chromosome. Clones of orfE overexpress RNase PH activity, verifying that orfE encodes this ribonuclease. We suggest that orfE be renamed rph.

Original languageEnglish (US)
Pages (from-to)5589-5591
Number of pages3
JournalJournal of bacteriology
Issue number17
StatePublished - 1991
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology


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