Escherichia coli isolates that carry vat, fyua, chua, and yfcv efficiently colonize the urinary tract

Rachel R. Spurbeck, Paul C. Dinh, Seth T. Walk, Ann E. Stapleton, Thomas M. Hooton, Lisa K. Nolan, Kwang Sik Kim, James R. Johnson, Harry L.T. Mobley

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

Extraintestinal Escherichia coli (ExPEC), a heterogeneous group of pathogens, encompasses avian, neonatal meningitis, and uropathogenic E. coli strains. While several virulence factors are associated with ExPEC, there is no core set of virulence factors that can be used to definitively differentiate these pathotypes. Here we describe a multiplex of four virulence factor-encoding genes, yfcV, vat, fyuA, and chuA, highly associated with uropathogenic E. coli strains that can distinguish three groups of E. coli: diarrheagenic and animal-associated E. coli strains, human commensal and avian pathogenic E. coli strains, and uropathogenic and neonatal meningitis E. coli strains. Furthermore, human intestinal isolates that encode all four predictor genes express them during exponential growth in human urine and colonize the bladder in the mouse model of ascending urinary tract infection in higher numbers than human commensal strains that do not encode the four predictor genes (P=0.02), suggesting that the presence of the predictors correlates with uropathogenic potential.

Original languageEnglish (US)
Pages (from-to)4115-4122
Number of pages8
JournalInfection and Immunity
Volume80
Issue number12
DOIs
StatePublished - Dec 14 2012

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ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Spurbeck, R. R., Dinh, P. C., Walk, S. T., Stapleton, A. E., Hooton, T. M., Nolan, L. K., Kim, K. S., Johnson, J. R., & Mobley, H. L. T. (2012). Escherichia coli isolates that carry vat, fyua, chua, and yfcv efficiently colonize the urinary tract. Infection and Immunity, 80(12), 4115-4122. https://doi.org/10.1128/IAI.00752-12