Escherichia coli 70 S ribosome at 15 Å resolution by cryo-electron microscopy: Localization of fMet-tRNA(f)(Met) and fitting of L1 protein

Arun Malhotra, Pawel Penczek, Rajendra K. Agrawal, Irene S. Gabashvili, Robert A. Grassucci, Ralf Jünemann, Nils Burkhardt, Knud H. Nierhaus, Joachim Frank

Research output: Contribution to journalArticle

159 Citations (Scopus)

Abstract

Cryo-electron microscopy of the ribosome in different binding states with mRNA and tRNA helps unravel the different steps of protein synthesis. Using over 29,000 projections of a ribosome complex in single-particle form, a three-dimensional map of the Escherichia coli 70 S ribosome was obtained in which a single site, the P site, is occupied by fMet-tRNA(f)(MET) as directed by an AUG codon containing mRNA. The superior resolution of this three-dimensional map, 14.9 Å, has made it possible to fit the tRNA X-ray crystal structure directly and unambiguously into the electron density, thus determining the locations of anticodon-codon interaction and peptidyltransferase center of the ribosome. Furthermore, at this resolution, one of the distinctly visible domains corresponding to a ribosomal protein, L1, closely matches with its X-ray structure.

Original languageEnglish
Pages (from-to)103-116
Number of pages14
JournalJournal of Molecular Biology
Volume280
Issue number1
DOIs
StatePublished - Jul 3 1998
Externally publishedYes

Fingerprint

RNA, Transfer, Met
Cryoelectron Microscopy
Ribosomes
Escherichia coli
Transfer RNA
Codon
Proteins
X-Rays
Peptidyl Transferases
Anticodon
Messenger RNA
Electrons

Keywords

  • Cryo-electron microscopy
  • L1 protein
  • Polypeptide exit tunnel
  • Ribosome structure
  • tRNA P-site

ASJC Scopus subject areas

  • Virology

Cite this

Escherichia coli 70 S ribosome at 15 Å resolution by cryo-electron microscopy : Localization of fMet-tRNA(f)(Met) and fitting of L1 protein. / Malhotra, Arun; Penczek, Pawel; Agrawal, Rajendra K.; Gabashvili, Irene S.; Grassucci, Robert A.; Jünemann, Ralf; Burkhardt, Nils; Nierhaus, Knud H.; Frank, Joachim.

In: Journal of Molecular Biology, Vol. 280, No. 1, 03.07.1998, p. 103-116.

Research output: Contribution to journalArticle

Malhotra, A, Penczek, P, Agrawal, RK, Gabashvili, IS, Grassucci, RA, Jünemann, R, Burkhardt, N, Nierhaus, KH & Frank, J 1998, 'Escherichia coli 70 S ribosome at 15 Å resolution by cryo-electron microscopy: Localization of fMet-tRNA(f)(Met) and fitting of L1 protein', Journal of Molecular Biology, vol. 280, no. 1, pp. 103-116. https://doi.org/10.1006/jmbi.1998.1859
Malhotra, Arun ; Penczek, Pawel ; Agrawal, Rajendra K. ; Gabashvili, Irene S. ; Grassucci, Robert A. ; Jünemann, Ralf ; Burkhardt, Nils ; Nierhaus, Knud H. ; Frank, Joachim. / Escherichia coli 70 S ribosome at 15 Å resolution by cryo-electron microscopy : Localization of fMet-tRNA(f)(Met) and fitting of L1 protein. In: Journal of Molecular Biology. 1998 ; Vol. 280, No. 1. pp. 103-116.
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