Erythropoietin producing cells for potential cell therapy

Tamer Aboushwareb, Fernanda Egydio, Lauren Straker, Kenneth Gyabaah, Anthony Atala, James J. Yoo

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Introduction: Anemia is an inevitable outcome of chronic renal failure due to the kidney's decreased ability to produce erythropoietin (EPO). We examined the feasibility of isolating and expanding EPO-producing cells for cell-based therapy. Materials and methods: Renal cells from 7- to 10-day-old mice were culture-expanded. The cells at each subculture stage were characterized for EPO expression, using immunocytochemistry, FACS, and Western Blot analysis, with EPO-specific antibodies. To assess the levels of EPO expression, cells incubated under normoxic and hypoxic conditions were analyzed. Results: Immunocytochemical analysis of the cultured renal cells expressed EPO at each subculture stage (P1-P3). Western Blot analysis of the detergent-solubilized cell extracts detected EPO (34 kDa) protein in the kidney cells of all passages tested. Conclusion: These results demonstrate that EPO-producing renal cells can be grown and expanded in culture. The cells stably expressed EPO at multiple subculture stages and they are able to form tissue in vivo. This study shows that EPO-producing cells may be used as a potential treatment option for anemia caused by chronic renal failure.

Original languageEnglish
Pages (from-to)295-300
Number of pages6
JournalWorld Journal of Urology
Volume26
Issue number4
DOIs
StatePublished - Jul 9 2008
Externally publishedYes

Fingerprint

Cell- and Tissue-Based Therapy
Erythropoietin
Kidney
Chronic Kidney Failure
Anemia
Western Blotting
Cell Extracts
Detergents
Cultured Cells
Immunohistochemistry
Antibodies

Keywords

  • Anemia
  • Cell therapy
  • Chronic kidney disease
  • Erythropoietin

ASJC Scopus subject areas

  • Urology

Cite this

Aboushwareb, T., Egydio, F., Straker, L., Gyabaah, K., Atala, A., & Yoo, J. J. (2008). Erythropoietin producing cells for potential cell therapy. World Journal of Urology, 26(4), 295-300. https://doi.org/10.1007/s00345-008-0301-0

Erythropoietin producing cells for potential cell therapy. / Aboushwareb, Tamer; Egydio, Fernanda; Straker, Lauren; Gyabaah, Kenneth; Atala, Anthony; Yoo, James J.

In: World Journal of Urology, Vol. 26, No. 4, 09.07.2008, p. 295-300.

Research output: Contribution to journalArticle

Aboushwareb, T, Egydio, F, Straker, L, Gyabaah, K, Atala, A & Yoo, JJ 2008, 'Erythropoietin producing cells for potential cell therapy', World Journal of Urology, vol. 26, no. 4, pp. 295-300. https://doi.org/10.1007/s00345-008-0301-0
Aboushwareb T, Egydio F, Straker L, Gyabaah K, Atala A, Yoo JJ. Erythropoietin producing cells for potential cell therapy. World Journal of Urology. 2008 Jul 9;26(4):295-300. https://doi.org/10.1007/s00345-008-0301-0
Aboushwareb, Tamer ; Egydio, Fernanda ; Straker, Lauren ; Gyabaah, Kenneth ; Atala, Anthony ; Yoo, James J. / Erythropoietin producing cells for potential cell therapy. In: World Journal of Urology. 2008 ; Vol. 26, No. 4. pp. 295-300.
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