Erythrocytic nucleoside diphosphokinase. IV. Evidence for electrophoretic heterogeneity

Yung Chi Cheng, R. P. Agarwal, R. E. Parks

Research output: Contribution to journalArticle

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Abstract

Human erythrocytic nucleoside diphosphokinase has been found to be heterogeneous by electrofocusing, electrophoresis, and ion-exchange chromatography. Several isozymes of the nucleoside diphosphokinase seem to be present. Six distinct peaks of enzymic activity, with isoelectric points from 5.4 to 8.3, were observed both in electrofocusing and in agarese electrophoresis. The electrophoretic patterns from the pooled blood of 100 persons or from single persons of different races showed no qualitative differences. Preliminary studies with the individual electrophoretic peaks revealed molecular weights ranging from 80,000 to about 100,000 indicating that aggregation is not a factor. Marked differences in the kinetic parameters were seen: e.g., Km values for ATP were 0.083 × 10-3 and 3.0 × 10-3 M for isozymes of pI = 7.3 and 6.3, respectively. Arrhenius plots were linear for isozymes of pI = 5.4, 5.8, 6.3, and 6.8 and were biphasic for isozymes of pI = 7.3 and 8.3. Present evidence indicates that the six electrophoretic peaks are distinctly different proteins.

Original languageEnglish
Pages (from-to)2139-2143
Number of pages5
JournalBiochemistry
Volume10
Issue number11
StatePublished - Dec 1 1971
Externally publishedYes

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Nucleoside-Diphosphate Kinase
Isoenzymes
Isoelectric Focusing
Electrophoresis
Arrhenius plots
Ion Exchange Chromatography
Isoelectric Point
Chromatography
Kinetic parameters
Ion exchange
Blood
Agglomeration
Adenosine Triphosphate
Molecular Weight
Molecular weight
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Cheng, Y. C., Agarwal, R. P., & Parks, R. E. (1971). Erythrocytic nucleoside diphosphokinase. IV. Evidence for electrophoretic heterogeneity. Biochemistry, 10(11), 2139-2143.

Erythrocytic nucleoside diphosphokinase. IV. Evidence for electrophoretic heterogeneity. / Cheng, Yung Chi; Agarwal, R. P.; Parks, R. E.

In: Biochemistry, Vol. 10, No. 11, 01.12.1971, p. 2139-2143.

Research output: Contribution to journalArticle

Cheng, YC, Agarwal, RP & Parks, RE 1971, 'Erythrocytic nucleoside diphosphokinase. IV. Evidence for electrophoretic heterogeneity', Biochemistry, vol. 10, no. 11, pp. 2139-2143.
Cheng, Yung Chi ; Agarwal, R. P. ; Parks, R. E. / Erythrocytic nucleoside diphosphokinase. IV. Evidence for electrophoretic heterogeneity. In: Biochemistry. 1971 ; Vol. 10, No. 11. pp. 2139-2143.
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