ERK 1/2 signaling pathway is involved in nicotine-mediated neuroprotection in spinal cord neurons

Michal J Toborek, Kwang Won Son, Anna Pudelko, Kelley King-Pospisil, Edward Wylegala, Andrzej Malecki

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Evidence indicates that agonists of neuronal nicotinic receptors (nAChRs), including nicotine, can induce neuroprotective and anti-apoptotic effects in the CNS. To study these mechanisms, the present study focused on nicotine-mediated modulation of the extracellular regulated kinase 1 and 2 (ERK1/2) pathway in cultured spinal cord neurons. Exposure to nicotine (0.1-10 μM) for as short as 1 min markedly upregulated levels of phosphorylated ERK1/2 (pERK1/2) and increased total ERK1/2 activity. Inhibition studies with mecamylamine and α-bungarotoxin revealed that these effects were mediated by the α7 nicotinic receptor. In addition, pre-exposure to U0126, a specific inhibitor of the ERK1/2 signaling, prevented nicotine-mediated anti-apoptotic effects. To indicate if treatment with nicotine also can activate ERK1/2 in vivo, a moderate spinal cord injury (SCI) was induced in rats using a weight-drop device and nicotine was injected 2 h post-trauma. Consistent with in vitro data, nicotine increased levels of pERK1/2 in this animal model of spinal cord trauma. Results of the present study indicate that the ERK1/2 pathway is involved in anti-apoptotic effects of nicotine in spinal cord neurons and may be involved in therapeutic effects of nicotine in spinal cord trauma.

Original languageEnglish
Pages (from-to)279-292
Number of pages14
JournalJournal of Cellular Biochemistry
Volume100
Issue number2
DOIs
StatePublished - Feb 1 2007
Externally publishedYes

Fingerprint

Nicotine
Neurons
Spinal Cord
Phosphotransferases
Spinal Cord Injuries
Nicotinic Receptors
Mecamylamine
Neuroprotection
Bungarotoxins
Therapeutic Uses
Rats
Animals
Animal Models
Modulation
Weights and Measures
Equipment and Supplies
Wounds and Injuries

Keywords

  • Apoptosis
  • Fatty acids
  • Nicotine
  • Signal transduction
  • Spinal cord injury

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

ERK 1/2 signaling pathway is involved in nicotine-mediated neuroprotection in spinal cord neurons. / Toborek, Michal J; Son, Kwang Won; Pudelko, Anna; King-Pospisil, Kelley; Wylegala, Edward; Malecki, Andrzej.

In: Journal of Cellular Biochemistry, Vol. 100, No. 2, 01.02.2007, p. 279-292.

Research output: Contribution to journalArticle

Toborek, Michal J ; Son, Kwang Won ; Pudelko, Anna ; King-Pospisil, Kelley ; Wylegala, Edward ; Malecki, Andrzej. / ERK 1/2 signaling pathway is involved in nicotine-mediated neuroprotection in spinal cord neurons. In: Journal of Cellular Biochemistry. 2007 ; Vol. 100, No. 2. pp. 279-292.
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